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Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes

A technology of differentially expressed genes and trimethylation, applied in the field of epigenetics research, can solve the problem of not finding specific biomarkers

Active Publication Date: 2013-07-17
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

A large number of literatures confirm that there are many studies on the pathogenesis of human MN, but the specific biomarkers of MN are still not found

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  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes
  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes
  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes

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Embodiment Construction

[0022] The analysis method and application of differentially expressed genes in the trimethylation state of membranous nephropathy will be described in further detail below, mainly in conjunction with the drawings and specific examples.

[0023] The method for analyzing differentially expressed genes in the trimethylation status of membranous nephropathy in an embodiment includes the following steps:

[0024] Step S110: Collect peripheral blood mononuclear cells from the patients with membranous nephropathy and the healthy control group respectively.

[0025] Step S120: Use 1% formaldehyde solution to cross-link the peripheral blood mononuclear cells respectively at room temperature, then wash with PBS solution, and then add 1M glycine solution to terminate cross-linking.

[0026] Step S130: Collect the cross-linked peripheral blood mononuclear cells, treat them with a homogenizer to lyse the nuclei, collect the lysate and dissolve and suspend it in a buffer, and use H3K9 trimethylated...

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Abstract

The invention relates to an analyzing method of membranous nephropathy tri-methyl status differential expression genes. Chromatin immunoprecipitation-high through-put (Chip-seq) sequencing is used for analyzing histone H3K9 tri-methyl status change of peripheral blood mononuclear cells (PBMCs) of a membranous nephropathy patient to obtain the membranous nephropathy tri-methyl status differential expression genes. By intensive researching the differential expression genes, pathogenesis of membranous nephropathy can be further clarified, and new paths are provided for diagnosis and treatment of membranous nephropathy. The analyzing method is reasonable and feasible in design, can effectively help to build a spectrum model of differential expression genes of membranous nephropathy, and related information, as the middle result, of membranous nephropathy can be achieved.

Description

Technical field [0001] The invention relates to the field of epigenetics research, in particular to an analysis method and application of differentially expressed genes in the trimethylation state of membranous nephropathy. Background technique [0002] Membranous nephropathy is characterized by diffuse thickening of the basement membrane and deposition of subepithelial immune complexes. The glomeruli also show unique morphological features: for example, thickened basement membrane, granular IgG in the glomerular capillary loops Deposition or electron dense deposits in the subepithelial tissue. Membranous nephropathy occurs all over the world, and there is no difference in race, age, or sex. So far, there are many studies on the pathogenesis of membranous nephropathy, but no specific biomarkers for membranous nephropathy have been found. So far, the changes in histone methylation status in membranous nephropathy are also unclear. [0003] The cellular and molecular understanding...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B40/08
Inventor 眭维国戴勇何慧燕
Owner 中国人民解放军联勤保障部队第九二四医院
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