Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes

A technology of differentially expressed genes and trimethylation, applied in the field of epigenetics research, can solve the problem of not finding specific biomarkers

Active Publication Date: 2015-06-03
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

A large number of literatures confirm that there are many studies on the pathogenesis of human MN, but the specific biomarkers of MN are still not found

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  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes
  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes
  • Analyzing method and application of membranous nephropathy tri-methyl status differential expression genes

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Embodiment Construction

[0022] The analysis method and application of differentially expressed genes in the trimethylation status of membranous nephropathy will be further described in detail below mainly in conjunction with the accompanying drawings and specific examples.

[0023] The method for analyzing the differentially expressed genes of the trimethylation state of membranous nephropathy in one embodiment comprises the following steps:

[0024] Step S110: collecting peripheral blood mononuclear cells from the patients with membranous nephropathy and the healthy control group respectively.

[0025] Step S120: the peripheral blood mononuclear cells were respectively cross-linked with 1% formaldehyde solution at room temperature, then washed with PBS solution, and then 1M glycine solution was added to terminate the cross-linking.

[0026] Step S130: collect the cross-linked peripheral blood mononuclear cells respectively, process and lyse the nuclei with a homogenizer, collect the lysates, dissolv...

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Abstract

The invention relates to an analyzing method of membranous nephropathy tri-methyl status differential expression genes. Chromatin immunoprecipitation-high through-put (Chip-seq) sequencing is used for analyzing histone H3K9 tri-methyl status change of peripheral blood mononuclear cells (PBMCs) of a membranous nephropathy patient to obtain the membranous nephropathy tri-methyl status differential expression genes. By intensive researching the differential expression genes, pathogenesis of membranous nephropathy can be further clarified, and new paths are provided for diagnosis and treatment of membranous nephropathy. The analyzing method is reasonable and feasible in design, can effectively help to build a spectrum model of differential expression genes of membranous nephropathy, and related information, as the middle result, of membranous nephropathy can be achieved.

Description

technical field [0001] The invention relates to the field of epigenetics research, in particular to an analysis method and application of differentially expressed genes in the trimethylation state of membranous nephropathy. Background technique [0002] Membranous nephropathy is characterized by diffuse thickening of the basement membrane and deposition of immune complexes in the subepithelium. Deposited or electron-dense material deposited in the subepithelial tissue. Membranous nephropathy occurs all over the world, without differences in race, age and gender. So far, there have been many studies on the pathogenesis of membranous nephropathy, but no specific biomarkers for membranous nephropathy have been found. To date, altered histone methylation status in membranous nephropathy is also unclear. [0003] Understanding the cellular and molecular aspects involved in the pathogenesis of membranous nephropathy (MN) comes from studies in the rat Heymann nephritis model, wh...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B40/08
Inventor 眭维国戴勇何慧燕
Owner 中国人民解放军联勤保障部队第九二四医院
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