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Cynoglossus semilaevis gender related gene, preparation method of recombinant protein thereof and application thereof

A technology of semi-smooth tongue sole and related proteins, applied in botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as failure to find

Active Publication Date: 2013-07-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the homologous genes of DMY were not found in some species closely related to medaka and other teleosts (Matsuda et al., 2003; Volff et al., 2003; Kondo et al., 2003), but there is no successful report on the cloning of sex-determining genes in other fish

Method used

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  • Cynoglossus semilaevis gender related gene, preparation method of recombinant protein thereof and application thereof
  • Cynoglossus semilaevis gender related gene, preparation method of recombinant protein thereof and application thereof
  • Cynoglossus semilaevis gender related gene, preparation method of recombinant protein thereof and application thereof

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Embodiment Construction

[0013] The method of the present invention will be further described below in conjunction with the accompanying drawings. However, the examples are for illustration only and are not limited thereto. For the experimental methods that do not indicate specific conditions in the following examples, generally, conventional conditions can be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run.

[0014] 1. Cloning and sequence of sex-related gene CSsr1 in half smooth tongue sole

[0015] The partial cDNA sequence of the sex-related gene CSsr1 of the half-smooth tongue sole was obtained through whole-genome sequencing, transcriptome and bioinformatics analysis, and then primers were designed to clone the full-length cDNA of the sex-related gene CSsr1 from the testis of the half-smooth tongue sole. The full-length sequence is SEQ ID NO :2, the encoded amino ...

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Abstract

The invention relates to a cynoglossus semilaevis gender related gene, wherein the amino acid sequence of the gene is SEQ ID NO: 1; and the nucleotide sequence for coding the gender related protein is SEQ ID NO: 2. The gene provided by the invention is closely related with sex reversal of cynoglossus semilaevis; the in-vitro recombinant protein is capable of obviously increasing the expression level of folliculostatin FST gene and inhibiting expression of female related genes P450, Fox12 and CTNNB1 in short term; and expression inhibition of genes is possible to reduce the sex reversal ratio of cynoglossus semilaevis; therefore, the cynoglossus semilaevis gender related gene has application potential in cynoglossus semilaevis gender control.

Description

technical field [0001] The invention belongs to the technical field of functional gene screening and application in aquatic biology technology, and in particular relates to the cloning, in vitro recombinant expression and activity analysis of the expression product of the sex-related gene CSsr1 of tongue sole. Background technique [0002] Cynoglossμs semilaevis (Cynoglossμs semilaevis) is a large bottom-dwelling fish with warm water in the coastal waters. Its meat is delicious and nutritious, and it is loved by consumers. It is one of the leading fish in mariculture in my country. However, the growth rate of female and male individuals of semi-smooth tongue sole is very different, and the growth rate of females is 2-4 times faster than that of males. Due to the slow growth and small size of male individuals, the cost of culture of tongue sole was increased, the production of culture was reduced, and the promotion of seedlings of tongue sole and the development of aquacultur...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N15/12C12N15/63
Inventor 胡乔木黄倢严芳
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI