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Method for rapidly breeding fritillaria pallidiflora by using tissue culture technique

A technology of Fritillaria Ili and tissue culture, which is applied in the field of rapid reproduction of Fritillaria Ili, and can solve the problems of not forming a breeding system, not having a large-scale reproduction method, and not many studies of Fritillaria Ili, etc. Shorten the incubation time, the effect of proper ratio

Active Publication Date: 2015-05-13
蚌埠行时知识产权运营有限公司
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Problems solved by technology

At present, the research on the tissue culture technology of Fritillaria has begun to take shape, but there are not many studies on Fritillaria Ili, and the breeding methods have not yet been scaled up, and a complete breeding system has not been formed.

Method used

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Embodiment Construction

[0017] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0018] A method for rapidly propagating Ili Fritillaria using tissue culture technology, comprising the following steps:

[0019] (1) Selection and disinfection treatment of explants: firstly select strong and mature fresh Ili Fritillaria bulb bulbs, peel off the dry wrinkled scales on the outermost layer of the bulbs and residual old roots at the base, rinse with running water for 30 minutes and put them in After soaking in clean water for 15 minutes, peel off the bulbs layer by layer, and then irradiate them under ultraviolet light for 20 minutes, then transfer them to the ultra-clean workbench, soak them in 70% ethanol for 15 seconds on the ultra-clean workbench, Then rinse with sterile water...

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Abstract

The invention discloses a method for rapidly breeding fritillaria pallidiflora by using a tissue culture technique. The method mainly comprises the steps of selecting and disinfecting explants, treating materials, inducing callus, carrying out subculture, carrying out rooting culture, seedling hardening, transplanting and the like, wherein the formation rate of the callus of the fritillaria pallidiflora is 93%, the proliferation rate is 6 times, the rooting percentage is 92%, the final survival rate is 90%, and the quality and the output of the fritillaria pallidiflora are both greatly improved; and moreover, the method simulates the growing environment of the fritillaria pallidiflora, the temperature is low in the culture process, chernozem is mainly taken as the culture medium, and the quality is greatly improved as the fritillaria pallidiflora grows in such a simulated wild environment.

Description

technical field [0001] The invention relates to a method for tissue culture of Fritillaria, in particular to a rapid propagation method suitable for Ili Fritillaria. Background technique [0002] Ili Fritillaria, a kind of Xinjiang Fritillaria, is mainly distributed in Xinjiang Habahe, Altay, Jimunai, Burqin, Nilek, Turks, Toli, Hot Spring, Bole, Huocheng, Bayinbuke and Gongnaisi and other areas, born in mountain grasslands at an altitude of 1500-2500 meters. Ili fritillary is a kind of plant that likes humidity and is cold-tolerant, and is suitable for growing in plateau areas. On the one hand, these places have a cool climate and more rainfall, which is suitable for the growth of fritillary. After planting, chernozem does not require fertilization for several years. Generally, it is not suitable to plant in places where the temperature is too high in the plains. This special environmental requirement determines that the reproduction of Ili Fritillaria is greatly limited;...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 陈勇明
Owner 蚌埠行时知识产权运营有限公司
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