Two-dimensional multi-channel chromatographic system and method for separating and screening strong antioxidant components of pinus koraiensis pinecone extract by using same

Abstract

The invention discloses a two-dimensional multi-channel chromatographic system and a method for separating and screening strong antioxidant components of a pinus koraiensis pinecone extract by using the same, and relates to a chromatographic system and a method for separating and enriching pinecone polyphenol by using the same. According to the system and the method, a novel two-dimensional multi-channel chromatographic system is combined with macrooprous resin for separating and screening the strong antioxidant components of the pinecone polyphenol. The two-dimensional multi-channel chromatographic system is characterized in that a chromatographic column is partitioned into an absorbing column and separating columns, so as to enable a polyphenol compound to be effectively and rapidly separated, and one absorbing column and a plurality of separating columns are adopted for achieving relatively effective separation. Compared with the single-column chromatograph, the two-dimensional multi-channel chromatographic system has the advantages that the polyphenol can be more effectively and rapidly separated, the separating speed can be remarkably increased by a plurality of times even about ten times, the separating efficiency can be remarkably improved by a plurality of times even about ten times, and functional components can be rapidly screened through combining with measurement of antioxidant indexes.

Description

technical field [0001] The invention relates to a chromatographic system and a method for separating and enriching pine cone polyphenols, in particular to a two-dimensional multi-channel chromatography system and a method for separating and enriching pine cone polyphenols. Background technique [0002] Korean pine is a perennial pinaceae plant mainly distributed in Northeast China and surrounding countries and regions. Studies on the extracts of Pinaceae plants have found that Pinaceae extracts have anti-oxidation, anti-tumor, anti-inflammation, anti-neurodegenerative diseases and anti-ultraviolet radiation effects. The most well-researched, Pycnogenol has become an internationally recognized additive in food, health products and cosmetics. [0003] Nowadays, we are faced with a lot of external toxic substances in our life. These toxic substances can cause a large amount of active oxygen in the body, and active oxygen can cause damage to the human body. Studies have found t...

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Problems solved by technology

However, traditional chromatography uses a single-column system, which takes a long time to operate and low efficiency

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