Two-dimensional multi-channel chromatographic system and method for separating and screening strong antioxidant components of pinus koraiensis pinecone extract by using same

An anti-oxidation component and chromatographic system technology, applied in the field of two-dimensional multi-channel chromatographic system and its separation and enrichment of pine cone polyphenols, can solve the problems of long operation time and low efficiency, and achieve the improvement of separation speed and efficiency , fast screening, high efficiency and fast separation effect

Inactive Publication Date: 2013-08-14
HARBIN INST OF TECH
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However, traditional chromatography uses a single-column sy

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  • Two-dimensional multi-channel chromatographic system and method for separating and screening strong antioxidant components of pinus koraiensis pinecone extract by using same

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[0015] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the technical solution of the present invention. in the scope of protection.

[0016] In this embodiment, the two-dimensional multi-channel chromatography system is used in combination with the macroporous resin to separate and screen the strong antioxidant components of the pine cone extract according to the following steps:

[0017] 1) Two-dimensional multi-channel chromatographic system: Usually the chromatographic column is a single-column chromatographic system, and the sample is loaded on the upper end of the single column, and the separation of the target product is achieved by using a suitable eluent. ...

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Abstract

The invention discloses a two-dimensional multi-channel chromatographic system and a method for separating and screening strong antioxidant components of a pinus koraiensis pinecone extract by using the same, and relates to a chromatographic system and a method for separating and enriching pinecone polyphenol by using the same. According to the system and the method, a novel two-dimensional multi-channel chromatographic system is combined with macrooprous resin for separating and screening the strong antioxidant components of the pinecone polyphenol. The two-dimensional multi-channel chromatographic system is characterized in that a chromatographic column is partitioned into an absorbing column and separating columns, so as to enable a polyphenol compound to be effectively and rapidly separated, and one absorbing column and a plurality of separating columns are adopted for achieving relatively effective separation. Compared with the single-column chromatograph, the two-dimensional multi-channel chromatographic system has the advantages that the polyphenol can be more effectively and rapidly separated, the separating speed can be remarkably increased by a plurality of times even about ten times, the separating efficiency can be remarkably improved by a plurality of times even about ten times, and functional components can be rapidly screened through combining with measurement of antioxidant indexes.

Description

technical field [0001] The invention relates to a chromatographic system and a method for separating and enriching pine cone polyphenols, in particular to a two-dimensional multi-channel chromatography system and a method for separating and enriching pine cone polyphenols. Background technique [0002] Korean pine is a perennial pinaceae plant mainly distributed in Northeast China and surrounding countries and regions. Studies on the extracts of Pinaceae plants have found that Pinaceae extracts have anti-oxidation, anti-tumor, anti-inflammation, anti-neurodegenerative diseases and anti-ultraviolet radiation effects. The most well-researched, Pycnogenol has become an internationally recognized additive in food, health products and cosmetics. [0003] Nowadays, we are faced with a lot of external toxic substances in our life. These toxic substances can cause a large amount of active oxygen in the body, and active oxygen can cause damage to the human body. Studies have found t...

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Application Information

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IPC IPC(8): B01D15/22A61K36/15
Inventor 王振宇李辉白海娜伊娟娟
Owner HARBIN INST OF TECH
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