Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of high-purity silk fibroin peptide

A high-purity, silk fibroin peptide technology, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low purity and achieve the effects of short process time, good application prospects, and simple preparation methods

Inactive Publication Date: 2013-08-14
SUZHOU UNIV
View PDF3 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As we all know, in order to improve the purity of the product, it is necessary to strengthen the removal of impurities during the preparation process. However, as far as the Chinese invention patent application CN1392265A is concerned, it uses a nanofiltration membrane for nanofiltration desalination, and its desalination rate reaches 98.39%. (See Table 3 in the specification), but its purity is still low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of high-purity silk fibroin peptide
  • Preparation method of high-purity silk fibroin peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of high-purity silk fibroin peptide, comprising the steps of:

[0023] (1) The following foot cocoons or waste silk are used as raw materials, and the volume concentration is 0.3% Na 2 CO 3 The solution is degummed and refined; after washing with water and drying, silk fibroin is obtained;

[0024] (2) The above silk fibroin was treated with CaCl 2 -H 2 O-C 2 h 5 OH ternary system dissolved; in moles, CaCl 2 :H 2 O: C 2 h 5 OH=1:8:2;

[0025] (3) After diluting the volume by 10 times with distilled water, carry out enzymolysis with neutral protease, the enzyme dosage is 150 activity units / g silk fibroin, the enzymolysis temperature is 45°C, and the time is 120min;

[0026] (4) Filter by electrodialysis with a current density of 20~25 mA / cm 2 , until the desalination rate reaches 90%;

[0027] (5) Filtration by dialysis: first use tap water as flowing water for dialysis, when the conductivity of the effluent from the dialysis membrane is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of high-purity silk fibroin peptide. The preparation method comprises the following steps of: (1) degumming and refining damaged cocoon or bavella silk serving as a raw material by using an Na2CO3 solution, and then washing and drying to obtain silk fibroin; (2) dissolving the silk fibroin by using a CaCl2-H2O-C2H5OH ternary system; (3) diluting, and then carrying out enzymolysis by using neutral proteinase; (4) filtering by adopting electrodialysis; (5) filtering by adopting dislysis; and (6) concentrating, carrying out spray drying, and sterilizing to obtain the high-purity silk fibroin peptide. According to the preparation method, tests prove that the desalinization ratio is 99.5%, and the content of peptide in a finally obtained silk fibroin peptide product is more than 94% and meets the use requirements of pharmaceuticals, cosmetics, medical materials, and the like, so that the problem of the field is solved, and the creativity is achieved.

Description

technical field [0001] The invention relates to a preparation method of high-purity silk fibroin peptide. Background technique [0002] At present, silk fibroin peptide is an advanced raw material for advanced natural nutritional skin care and hair care products. Adding silk fibroin peptide to skin care products can increase the vitality of skin cells and have the effect of sunscreen, whitening and moisturizing; adding silk peptide to hair care products , can form a protective film on the surface of the hair, increase the elasticity of the hair, nourish and moisturize, and can repair the damage caused by perm and hair dyeing. In addition, silk fibroin peptide also has important utilization value in biopharmaceuticals, external medical materials and functional health food, and can be made into liver-protecting drugs, diabetes drugs, Alzheimer's drugs, etc.; it can be applied It is used in the manufacture of medical supplies such as contact lenses, artificial skin and wound m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 盛家镛邢铁玲陈国强
Owner SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com