Fluoride modification based dendrimer gene transfer vector as well as preparation method and application thereof

A gene transfection carrier and dendrimer technology, which is applied in the field of polymer chemistry and biomaterials, can solve the problems of ineffective antiserum transfection, and achieve the effects of saving vectors and DNA, reducing costs, and simple synthesis steps

Inactive Publication Date: 2013-09-11
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This can effectively solve the problem that current commercialized transfection reagents cannot effectively resist serum transfection (such as Lipofectamine2000, Fugene, etc.)

Method used

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  • Fluoride modification based dendrimer gene transfer vector as well as preparation method and application thereof
  • Fluoride modification based dendrimer gene transfer vector as well as preparation method and application thereof
  • Fluoride modification based dendrimer gene transfer vector as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: A new material for gene transfection based on trifluoroacetylated fifth-generation polyamide-amine dendrimer was prepared at a ratio of 1:32.

[0054] Taking the material based on covalently linked trifluoroacetylated fifth-generation polyamide-amine dendrimers as an example, its structure is as follows:

[0055]

[0056] Among them, the theoretical expectation of a is 32, and G5 is the fifth-generation polyamide-amine dendrimer, wherein the central core M is ethylenediamine, and the terminal group is 128 primary amine groups. Its structural formula is as follows:

[0057]

[0058] Preparation and synthesis method: take 50 mg of the fifth-generation polyamide-amine dendrimer, dissolve it in 2 ml of methanol solution, slowly add 9.25 microliters of triethylamine dropwise to the methanol solution in which the dendrimer is dissolved , and then dropwise added 1 ml of trifluoroacetic anhydride methanol solution in which 7.68 microliters were dissolved, and a...

Embodiment 2

[0069] Example 2: A new material for gene transfection based on trifluoroacetylated fifth-generation polyamide-amine dendrimer was prepared at a ratio of 1:64.

[0070] Taking the material based on the fifth-generation polyamide-amine dendrimer covalently attached to the surface with trifluoroacetylation modification as an example, its structure is as follows:

[0071]

[0072] Among them, the theoretical expectation of a is 64, and G5 is a fifth-generation polyamide-amine dendrimer, wherein the central core M is ethylenediamine, and the terminal group is 128 primary amine groups. Its structural formula is as follows:

[0073]

[0074] Preparation and synthesis method: Take 50 mg of the fifth-generation polyamide-amine dendrimer, dissolve it in 2 ml of methanol solution, slowly add 18.57 microliters of triethylamine dropwise to the methanol solution in which the dendrimer is dissolved , then slowly add 1 milliliter of trifluoroacetic anhydride methanol solution with 15.4...

Embodiment 3

[0085] Example 3: A new material for gene transfection based on pentafluoropropionylated fifth-generation polyamide-amine dendrimer was prepared at a ratio of 1:32.

[0086] Taking the material based on covalently linked pentafluoropropionylated fifth-generation polyamide-amine dendrimers as an example, its structure is as follows:

[0087]

[0088] Among them, the theoretical expectation of a is 32, and G5 is the fifth-generation polyamide-amine dendrimer, whose central core is ethylenediamine, and the terminal group is 128 primary amine groups. Its structural formula is as follows:

[0089]

[0090] Preparation and synthesis method: take 50 mg of the fifth-generation polyamide-amine dendrimer, dissolve it in 2 milliliters of methanol solution, add 9.25 microliters of triethylamine dropwise to the methanol solution in which dendrimers are dissolved, and then Add 1 ml of 10.95 microliters of pentafluoropropionic anhydride methanol solution dropwise to the solution, and a...

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Abstract

The invention provides a fluoride modification based dendrimer gene transfer vector. The vector comprises a dendrimer skeleton and a fluorochemical functional group, wherein the fluorochemical functional group is covalently connected on the surface of a dendrimer; the dendrimer comprises a polyamidoamine dendrimer and a poly(propyleneimine) dendrimer. The invention also provides a preparation method of the gene transfer vector and application of the gene transfer vector as a nucleic acid molecule conveying vector. The invention also provides a compound comprising the gene transfer vector. The gene transfer vector is simple and efficient, has high yield, can reduce low nitrogen / phosphorus ratio and achieve an efficient transfer effect in the cell transfer process, is low in cost, has low cytotoxicity, can effectively and safely convey gene molecules to cells and has the advantages of efficiency, low toxicity, low price, simpleness in synthesis and the like.

Description

technical field [0001] The invention relates to the technical fields of polymer chemistry and biomaterials, in particular to a gene transfection carrier based on a fluoride-modified dendrimer and a preparation method and application thereof. Background technique [0002] Gene transfection refers to the process of introducing and expressing foreign genes into cells or tissues. This technique is widely used in scientific research and disease treatment. In this process, gene transfection vector plays a very important role. In general, gene transfection vectors can be mainly divided into two categories: viral vectors and non-viral vectors. Viral transfection methods can be used to obtain cells or tissues that express foreign DNA efficiently for a long time, but viral vectors have problems such as cell line applicability, limited ability to carry genes, and easy to cause cellular immune responses and potential safety hazards. At present, safe, low toxicity, and easy-to-operate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/63C08G83/00
Inventor 程义云王铭明刘红梅
Owner EAST CHINA NORMAL UNIV
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