Rapid detection method of real-time PCR for banana bunchy top virus

Abstract

The invention belongs to the technical field of biologics, and discloses a real-time PCR (Polymerase Chain Reaction) rapid detection method which is used for detecting banana bunchy top viruses and which is based on a Taqman probe. The method is characterized by comprising the following steps of: (1) rapidly preparing a detection sample; and (2) carrying out real-time PCR amplification so as to obtain a result. The invention aims at overcoming the defects in the prior art and providing a method which is simple and capable of detecting viruses in banana plants without obvious susceptible symptoms, and detecting whether Pentalonia nigronervosa carries the viruses as well; and by utilizing the method, the cross contamination of samples is effectively reduced, and the detection is high in efficiency and accurate.

Description

technical field [0001] The invention belongs to the application field of agricultural biotechnology, and relates to a real-time PCR detection method for detecting banana bunchy top virus and a kit established according to the method. Background technique [0002] Banana is an important economic crop in the south of my country. It is the pillar industry of the fruit industry in some cities in the south and an important export agricultural product. It is exported to Japan and other countries. At present, banana virus disease has always been one of the main limiting factors in my country's banana production and the export of banana tissue cultured seedlings. Banana bunchy top is one of the most serious diseases of bananas. [0003] Banana bunchy top virus (BBTV) is the pathogen of banana bunchy top disease, which causes bunchy tops and obvious dwarfing of plants, yellowing of the outer edges of leaves, and dark green stripes on leaf veins, petioles and pseudostems. The transm...

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Problems solved by technology

[0005] In the existing detection technology, the commonly used method is enzyme-linked immunosorbent assay (ELISA), but asymptomatic banana plants, banana seedlings, especially tissue-cultured seedlings, because the plants are young and the virus content in the body is very low, the ELISA method is often used. There will be missed and false detections; the detection method of ordinary PCR is more than 1000 times more sensitive than the ELISA method, which is very suitable for detecting low-concentration virus samples. It takes a long time and high cost, and it is easy to cause cross-contamination of samples, and the amount of virus carried by susceptible samples cannot be quantified

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