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Application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus

A technology of mesenchymal stem cells and lupus erythematosus, which is applied in the field of preparation of stem cells to treat diseases, can solve problems such as difficulty in obtaining materials, and achieve the effects of high activity, low preparation cost, and low risk of immune response

Active Publication Date: 2014-12-17
胡祥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, embryonic stem cell research has always been a controversial field because of ethical issues, and its source is restricted by ethics and morality, making it difficult to obtain materials
This study suggests that the abnormal secretion of cytokines may be one of the reasons for the damage of the hematopoietic system and the disorder of the immune system in SLE, but the specific mechanism of the cytokine secretion defects of bone marrow MSCs in SLE patients needs further study

Method used

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  • Application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus
  • Application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus
  • Application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 1. Stripping of umbilical cord Wharton's jelly

[0070] Immediately after the fetus was delivered, the umbilical cord was ligated and cut according to the obstetrical routine, the umbilical cord was washed with normal saline, and then disinfected with medical alcohol, and the umbilical cord was stored in the umbilical cord preservation solution at a constant temperature of 2-8°C.

[0071] The obtained umbilical cord was rinsed with 0.9% sodium chloride injection, and repeated 2 to 3 times to remove blood stains. The whole umbilical cord was sterilized by immersing it in 75% ethanol. Sodium chloride injection was washed repeatedly to remove residual ethanol. Use sterile surgical scissors to cut the umbilical cord into several sections of about 2 to 5 cm, and remove the congestion and clots in the blood vessels of the small section of the umbilical cord. Remove two arteries and one vein from the umbilical cord. The white connective tissue located between the amniotic m...

Embodiment 2

[0084] 1. Passage and culture of P0 generation cells

[0085] The cell suspension was centrifuged at 300g for 10min. Discard the supernatant, resuspend the cells by gently pipetting with fresh medium, and follow the 5×10 3 piece / cm 2 Density inoculation and passage. Each T175 flask was inoculated with cell suspension, and fresh medium was added. Place in a carbon dioxide constant temperature and humidity incubator to start culturing. Conditions: 37.0±0.5°C, carbon dioxide volume fraction 5.0±0.2%.

[0086] 2. Cell passage

[0087] Cells were cultured for 72±24 hours, and after the cell confluence was observed under an inverted microscope to reach 80%-90%, they could be digested and harvested to obtain P1 generation cells.

[0088] 2.1 Digestion

[0089] Transfer the old culture medium to a sterile container (such as a T175 culture bottle) for later use. Rinse the culture bottle gently with an appropriate amount of 0.9% sodium chloride injection, and discard the washing...

Embodiment 3

[0110] 1. Recovery of cells

[0111] Take out the frozen cells from the liquid nitrogen tank, put them on the recovery rack in the electric heating constant temperature water tank (the water temperature is kept at about 40.0°C), and shake quickly to rewarm. Resuscitation is complete when the cell suspension changes from solid to liquid.

[0112] 1.1 Cell washing:

[0113] Combine the cell suspension into a centrifuge tube. Add pre-cooled 0.9% sodium chloride injection, blow and wash the pipette, constant volume, mix, centrifuge, 300g, 10min.

[0114] 1.2 Sampling counting and washing:

[0115] Discard the washing supernatant, resuspend the cell pellet in an appropriate amount of pre-cooled 0.9% sodium chloride injection, combine it into a centrifuge tube, send the sample to detect the total number of cells and cell viability, centrifuge the cell suspension at 300g, 10min, discard the upper cleared to obtain P3 generation cells.

[0116] 1.3 Passage and cultivation:

[01...

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Abstract

The invention provides an application of umbilical cord mesenchymal stem cells in preparation of formulation for treating lupus erythematosus, in particular to an application in preparation of formulation for treating recurrent systemic lupus erythematosus. The invention further provides a method of preparing umbilical cord mesenchymal stem cells of formulation capable of being used for treating the lupus erythematosus and a formulation composition having the stem cells. The umbilical cord mesenchymal stem cells prepared by the invention have the advantages of low immunoreaction risk, high activity, low residue, and the like, and the preparation cost is low.

Description

technical field [0001] The invention relates to the field of stem cell preparation for treating diseases. Specifically, the present invention provides a use of umbilical cord mesenchymal stem cells in preparing a preparation for treating lupus erythematosus. Background technique [0002] Autoimmune diseases refer to diseases caused by the body's immune response to self-antigens, resulting in damage to its own tissues. Autoimmune diseases can be divided into organ-specific autoimmune diseases and systemic autoimmune diseases. In organ-specific autoimmune diseases, the pathological damage and dysfunction of tissues and organs are limited to a certain organ targeted by antibodies or sensitized lymphocytes, mainly including chronic lymphatic thyroiditis, hyperthyroidism, insulin-dependent diabetes mellitus, myasthenia myasthenia Weakness, chronic ulcerative colitis, pernicious anemia with chronic atrophic gastritis, pulmonary hemorrhage nephritic syndrome, multiple sclerosis, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K35/44A61P19/04A61P37/02A61K35/28
Inventor 胡祥刘沐芸李陶罗朝霞李婵汪照静孟繁伟
Owner 胡祥
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