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Kit for verifying warehouse booklice based on specific primer

A storage book louse and kit technology are applied in the field of identification or auxiliary identification of storage book louse kits, and can solve problems such as heavy workload and the like

Active Publication Date: 2015-02-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The result of restriction fragment length polymorphism PCR technology is relatively accurate, but the workload of screening the required restriction endonucleases is relatively large
There is no systematic report on the identification of 10 common storage molecular techniques in the world

Method used

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  • Kit for verifying warehouse booklice based on specific primer
  • Kit for verifying warehouse booklice based on specific primer
  • Kit for verifying warehouse booklice based on specific primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1. Design and synthesis of specific primers for 10 kinds of storage booklice

[0083] 1. Acquisition of ITS2 rDNA sequences of 10 species of stored booklice

[0084] Experimental materials: Involving 10 kinds of storage booklice, specifically bookhopper booklice, booklice insectophilus, colorless booklice, small-eyed booklice, nibbling booklice, brown booklice, red booklice, skinny booklice, The hypocrisy booklice and the tricolor booklice. Among them, the following geographical populations are involved in the bookhopper: Guangxi population, Henan population, Chongqing population, American population, Czech population in Prague, Beijing population, Guangzhou population, and German population; , Guangxi population, Czech population, Shandong population; colorless booklice involves the following geographical populations: Yunnan population, Chongqing population, and American population; small-eyed booklice involves the following geographical populations: Shandong ...

Embodiment 2

[0114] Example 2. Specific detection of 10 kinds of storage book lice ITS2 rDNA sequence-specific primers

[0115] 1. Experimental materials

[0116] Involves 10 species of storage booklice, specifically booklice booklice, booklice entomophilus, colorless booklice, small-eyed booklice, nibbling booklice, brown booklice, red booklice, skinny booklice, hypocrisy booklice and tricolor booklice. Among them, the following geographical populations are involved in the bookhopper: Guangxi population, Henan population, Chongqing population, American population, Czech population in Prague, Beijing population, Guangzhou population, and German population; , Guangxi population, Czech population, Shandong population; colorless booklice involves the following geographical populations: Yunnan population, Chongqing population, and American population; small-eyed booklice involves the following geographical populations: Shandong Taian population, Shandong Caoxian population, Zhejiang populatio...

Embodiment 3

[0146] Example 3. Sensitivity detection of 10 kinds of stored book lice ITS2 rDNA sequence-specific primers

[0147] 1. Experimental materials

[0148] Involves 10 species of storage booklice, specifically booklice booklice, booklice entomophilus, colorless booklice, small-eyed booklice, nibbling booklice, brown booklice, red booklice, skinny booklice, hypocrisy booklice and tricolor booklice. Among them, the geographical population of Booklice curlyphilus is specifically the population of Guangxi; the geographical population of Booklice entomophagous is specifically the population of Chongqing; the geographical population of Booklice without color is specifically the population of Chongqing; the geographical population of Booklice microphthalmia is specifically the population of Zhejiang; Geographical populations of the bookhopper are specified in the Czech population; geographic populations of the brown booklice are specified in the American population; geographic populatio...

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Abstract

The invention discloses a kit for verification or assisted verification of a warehouse booklice. The kit provided by the invention comprises at least one pair of the following ten primer pairs: a primer pair 1 (sequences 1 and 2), a primer pair 2 (sequences 3 and 4), a primer pair 3 (sequence 5 and 6), a primer pair 4 (sequences 7 and 8), a primer pair 5 (sequence 9 and 10), a primer pair 6 (sequence 11 and 12), a primer pair 7 (sequence 13 and 14), a primer pair 8 (sequence 15 and 16), a primer pair 9 (sequence 17 and 18) and a primer pair 10 (sequence 19 and 20). Experiments prove that the kit for verifying the warehouse booklice based on specific primers provided by the invention can be used for verifying 10 types of booklices in the world, is strong in specificity, simple to operate, high in repeatability, high and stable in sensitivity, and is an ideal method for verifying the booklice.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a kit for identifying or assisting in identifying stored booklice. Background technique [0002] Booklice, also known as paper lice and rice lice, belongs to the order Psocoptera, the family Liposcelidiae, and the genus Liposcelis, and is a common pest in the protection of stored grains in the world. This genus of insects is widely distributed in the world. Currently, more than 120 species are known. There are 27 species of booklice recorded in my country. Among them, the booklice related to grain storage mainly include booklice entomophilus, booklice curl, colorless booklice, Booklice microphthalmia, booklice nibbling, brown booklice, red booklice, booklice pipietii, false booklice, tricolor booklice. [0003] Booklice are the most economically important group of rodent insects. Booklice are tiny (adults are about 1 mm long), have a wide range of occurrence, and have strong mobility....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 李志红崔冰艺
Owner CHINA AGRI UNIV
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