Method for preparing rhesus monkey type 2 diabetic model

A technology of type 2 diabetes and rhesus monkeys, which is applied in the field of preparation of rhesus monkey type 2 diabetes models, can solve the problems of high cost, scarcity, and immature technology of transgenic technology, and achieve strong repeatability and drug administration The effect of simple method

Inactive Publication Date: 2013-09-25
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chance of screening spontaneous diabetic animals in primates is very small, and the number is rare, and transgenic technology in primates is not yet a mature technology, and the cost is high

Method used

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  • Method for preparing rhesus monkey type 2 diabetic model
  • Method for preparing rhesus monkey type 2 diabetic model
  • Method for preparing rhesus monkey type 2 diabetic model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation of rhesus monkey type 2 diabetes syndrome model of the present invention

[0031] 1 Experimental materials and instruments

[0032] 1.1 Experimental materials

[0033] Eight 6-month-old rhesus monkeys (half male and half male), free from herpes B virus, simian retrovirus, simian leukemia virus and immunodeficiency virus infection, were purchased from Chengdu Ping An Animal Breeding Base.

[0034] Experimental group: STZ group (HF+STZ group, 4 rats) was given a high-fat and high-cholesterol diet for 24 months to induce type 2 diabetes by intravenous injection of small doses of streptozotocin, and the high-fat group (HF group, 4 rats) was only given high Cholesterol diet feeding.

[0035] Control group: monkeys were given standard monkey feed

[0036] Feeding method: 2 times a day, the feeding amount is 0.3-0.4kg / time per bird.

[0037] Feed formula:

[0038] Control group: monkey standard monkey feed——commercialized monkey pellet feed (p...

Embodiment 2

[0085] Embodiment 2 screens the medicine for the treatment of type 2 diabetes with the model of the present invention

[0086] A, the rhesus monkey type 2 diabetes model established according to the method of Example 1;

[0087] b. administering the drug candidate to an animal model;

[0088] c. Observe the effect of candidate drugs on various indicators of type 2 diabetes, and evaluate potential drugs for treating type 2 diabetes.

[0089] In summary, the modeling method of the present invention, on the basis of high-fat and high-cholesterol feed supplemented with a small dose of STZ, can not only cause some β-cell cell damage, meet the necessary conditions for type 2 diabetes, but also induce insulin resistance through lipid metabolism disorder, Forming a relatively stable and standardized rhesus monkey T2DM model in a relatively short period has important practical application value.

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Abstract

The invention discloses a method for preparing a rhesus monkey type 2 diabetic model. The method includes the steps: (1) feeding rhesus monkeys with high fat and high cholesterol feed twice a day; (2) applying streptozocin with the dosage of 25mg/Kg to the monkeys once a week and continuously applying the streptozocin twice after 24 months. Each rhesus monkey is fed with 0.3-0.4kg of the feed every time, and the feed comprises raw materials including, in weight ratio, 78 parts of standard monkey feeds, 15 parts of animal fat, 5 parts of sugar and 2 parts of cholesterol. By a combined modeling mode of long-term feeding of the high fat and high cholesterol feed and intravenous injection of the small-dosage streptozocin, the rhesus monkey type 2 diabetic model is successfully induced.

Description

technical field [0001] The invention relates to a method for preparing a rhesus monkey type 2 diabetes model. Background technique [0002] In recent years, the incidence of diabetes worldwide has increased rapidly. According to the latest data released by WHO, the number of diabetic patients in the world has exceeded 177 million, and it is expected to reach 370 million by 2025. The incidence of diabetes in my country is also increasing at an alarming rate. A recent epidemiological survey by the Diabetes Branch of the Chinese Medical Association found that the incidence of diabetes in the country has rapidly increased from 4% in 2002 to 11% now, and there are still 15% People with abnormal blood sugar. At present, the number of diabetic patients in my country has reached 91 million, ranking first in the world. Among diabetic patients, more than 90% are type 2 diabetes mellitus (Type2Diabetes Mellitus, T2DM). Continuous exploration of new treatment models for T2MD and devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A23K1/18A61K31/7008A61P3/10
CPCA01K67/02A01K67/027A01K2207/25A01K2227/106A01K2267/0362A23K50/00A61P3/10
Inventor 陈又南程惊秋陆燕蓉刘敬平李新丽杨光张杰廖光能李宏霞王莉袁玉佳何斯荣王成世
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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