Preparation method of a kind of animal model of phlegm and blood stasis mixed ovarian cyst

A technology of phlegm and blood stasis interlocking type and ovarian cyst, which can be applied to preparations for in vivo experiments, medical raw materials derived from mammals, medical preparations containing active ingredients, etc. Nodal ovarian cysts and other problems, to achieve the effect of strong reproducibility and simple administration method

Inactive Publication Date: 2016-03-16
CHENGDU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no animal model of ovarian cyst with phlegm and stasis, and it is difficult to effectively screen new drugs

Method used

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  • Preparation method of a kind of animal model of phlegm and blood stasis mixed ovarian cyst
  • Preparation method of a kind of animal model of phlegm and blood stasis mixed ovarian cyst
  • Preparation method of a kind of animal model of phlegm and blood stasis mixed ovarian cyst

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Effect test

Embodiment 1

[0024] Embodiment 1 The establishment method of the animal model of phlegm and blood stasis intermingled ovarian cyst of the present invention

[0025] 1 Materials and methods

[0026] 1.1 Experimental animals

[0027] Healthy SD clean female non-pregnant rats, weighing 150±10g. Provided by the Experimental Animal Center of Sichuan University (animal qualification certificate number: SCXK Chuan 2008-24), it will be used after 1 week of adaptive feeding.

[0028] 1.2 Main reagents

[0029] Estradiol Benzoate Injection, the concentration of Estradiol Benzoate is 5mg / ml (Tianjin Jinyao Amino Acid Co., Ltd., National Drug Approval H12020529); Olive Oil (Chengdu Kelong Chemical Preparation Factory); Cholesterol (Shanghai Boao Biotechnology Co., Ltd.); Medicinal Tween-80 (Nanjing Well Chemical Co., Ltd.); Rapidly Improved Papanicolaou Staining Solution (Nanjing Jiancheng Technology Co., Ltd.); Estradiol EISA Kit (Shanghai Jingyan Biotechnology Co., Ltd.) ;Total cholesterol (CHO)...

Embodiment 2

[0068] Embodiment 2 uses the model of the present invention to screen the medicine for the treatment of phlegm and stasis intermingled ovarian cyst

[0069] a, the phlegm and stasis intermingled ovarian cyst established according to the method of Example 1;

[0070] b. administering the drug candidate to an animal model;

[0071] c. Observe the effects of candidate drugs on various indicators of ovarian cysts with phlegm and stasis, and evaluate potential drugs for treating ovarian cysts with phlegm and stasis.

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Abstract

A method for preparing a stasis-phlegm-type ovarian cysts (OC) animal model, comprising the following steps: (1) applying estradiol benzoate solution to a rat in estrus, the application dosage being 1.5 ml / 100 g once every five days four consecutive times, and the concentration of the estradiol benzoate solution being 3.75 mg / ml; (2) from the day when the estradiol benzoate solution is applied for the first time, performing intragastric administration with 1 ml / 100 g high-fat milk once a day twenty consecutive times, each 100 ml high-fat milk comprising the following raw materials by weight: 20 g cholesterol, 3 g sodium cholate, 35 g animal fat, 20 ml propylene glycol, and 10 ml polysorbate-80, and the balance being water.

Description

technical field [0001] The invention relates to a method for preparing an animal model of ovarian cyst with mixed phlegm and blood stasis. Background technique [0002] Ovarian cysts (ovariancysts, OC) refer to the formation of lumps in or on the surface of the ovary. The substance in the lump is usually liquid, sometimes it may be solid, or a mixture of liquid and solid. Ovarian cyst is the most common gynecological disease, which can occur in any female age, especially in women of childbearing age. There are many aspects to the pathogenesis of ovarian cysts. The main reason is that hypothalamic-pituitary-ovarian dysfunction leads to pathological gonadotropin secretion, which affects the production, development, and release of follicles, and eventually forms ovarian cysts. Ovarian cysts belong to the category of "accumulation", "sickness" and "intestinal pain" in traditional Chinese medicine. Its formation is mostly caused by the invasion of six evils, damage of seven emot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/35A61K49/00A61K31/565A61K31/575
CPCA61K49/0008A01K67/027A01K2207/20A01K2227/105A01K2267/035A61K35/12
Inventor 张仲林凌保东钟玲臧志和袁明勇辛志伟彭延娟颜晓燕盛艳梅
Owner CHENGDU MEDICAL COLLEGE
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