Method for aided identification of fat tail properties of sheep and special molecular marker used therein
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A technology of lipid tail traits and molecular markers, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., and can solve problems such as extinction, unpopularity, and uneconomical principles of tail fat deposition
Active Publication Date: 2013-09-25
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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[0004] With the continuous improvement of people's living standards, the construction of herdsmen's settlements, the continuous improvement of pasture planting and other supporting technologies, and people's high concern about cardiovascular diseases and other factors, fat-rump sheep breeds are becoming less and less popular. The "life-saving" effect of fat is negligible, and there are the following limitations at present: when mutton is sold, most of the tail fat is removed, thereby reducing its economic value; based on health considerations, people's diet tends to "like thinness and dislike fat" ; From the perspective of feeding costs, the forage material consumed by 1 kg of fat is equivalent to the forage material required by 2 kg of lean meat, and excessive tail fat deposition is not in line with economic principles
If it is not improved and genetically protected, it will gradually lose its excellent genetics and face the danger of extinction
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Embodiment 1
[0025] Embodiment 1, design and synthesis of specific primer pair
[0026] Based on a large number of sequence alignments, analysis and preliminary experiments, design specific primer pairs as follows:
[0027] F (upstream primer, sequence 1 of the sequence listing): 5'-AGGTAGGGAGATTATCCAGGGAG-3';
[0028] R (downstream primer, sequence 2 of the sequence listing): 5'-ACAGCAGGGTTCTCTGCTCAGAG-3'.
Embodiment 2
[0029] Example 2, Application of specific primers to identify the relationship between polymorphism and traits
[0030] 1. Extract the genomic DNA of the sample to be tested, use the Nanodrop2000 instrument to detect the purity and concentration of the DNA sample, select the DNA sample with an OD260 / OD280 ratio between 1.7-1.9, and add ddH 2 O was diluted to 100ng / L and stored frozen at -20°C.
[0031] 2. Using the genomic DNA extracted in step 1 as a template, the specific primer pair designed in Example 1 is used for PCR amplification.
[0032] PCR reaction system (25μL): template 100ng, 10×PCR buffer 2.5μL, 2.5mmol / L MgCl 2 , 150mol / L dNTPs, upstream primer 0.2pmol / L, downstream primer 0.2pmol / L, Taq DNA polymerase 2.5U, add ddH 2 0 to 25 μL.
[0033] PCR reaction conditions: pre-denaturation at 94°C for 5 minutes; 35 cycles of 94°C for 30s, 60°C for 30s, and 72°C for 30s; extension at 72°C for 10 minutes; storage at 4°C.
[0034] 3. Take the PCR amplification product o...
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Abstract
The invention discloses a method for aided identification of fat tail properties of sheep and a special molecular marker used therein. The invention provides the method used for aided identification of fat tail properties of a to-be-detected sheep colony. The method comprises the following steps: (1) randomly drawing a to-be-detected sample with statistical significance from the to-be-detected sheep colony; (2) detecting the T / C ratio of site 59383635 of an X chromosome of the to-be-detected sample; and (3) carrying out evaluation according to the standards that the to-be-detected sheep colony is a fat rump type colony if the T / C ratio is greater than 5, the to-be-detected sheep colony is a slim tail type colony if the T / C ratio is smaller than 0.5 and the to-be-detected sheep colony is a short fat tail type colony if the T / C ratio is in a range of 1 to 2. Experimental results of the invention show that distribution of the site 59383635 of the X chromosome of sheep in a fat tail (rump) type sheep colony and a slim tail type sheep colony is greatly different and the SNP site can be used as an ideal molecular marker for breeding of a low fat sheep variety.
Description
technical field [0001] The invention relates to a method for assisting the identification of fat tail traits of sheep and a special molecular marker thereof. Background technique [0002] Sheep are the earliest domesticated herbivorous livestock. Animal archaeologists have found that as early as 9,000 years ago in the Neolithic Age, domestic sheep had appeared in northern Iraq and the Middle East adjacent to Iran. With continuous evolution and selection, domestic sheep have the ability to adapt to the geographical environment of lack of nutrition and extremely harsh climatic conditions, and are widely distributed in vast areas ranging from low-lying swamps, plains, deserts to plateaus and high cold. [0003] The suet tail (rump) trait is a biological trait necessary for survival in adversity, and it played an irreplaceable role in a specific historical period. The formation of fat-tailed sheep breeds can be traced back to the Uruk Stone Age in Iraq 5,000 years ago. Iranian ...
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