Air exhausting device of free-flow electrophoresis separation cavity and implementation method of air exhausting device

A separation device, electrophoresis separation technology, applied in the direction of separation method, material separation, measuring device, etc., can solve the problem of air discharge, etc., and achieve the effect of low equipment cost, reasonable design and simple operation

Active Publication Date: 2013-10-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the free-flow separation chamber had to adjust the angle of the separation chamber before use, and repeatedly adjusted back and forth, left and right to remove the air in the separation chamber, which was a very troublesome process.

Method used

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  • Air exhausting device of free-flow electrophoresis separation cavity and implementation method of air exhausting device
  • Air exhausting device of free-flow electrophoresis separation cavity and implementation method of air exhausting device
  • Air exhausting device of free-flow electrophoresis separation cavity and implementation method of air exhausting device

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Embodiment

[0017] The first embodiment of the present invention is figure 1 , image 3 and Figure 4 As shown, the present invention includes a buffer liquid storage bottle 3, a buffer liquid pump 2, a manual air cylinder 1, a hose clamp 11, a rubber hose 12, a gas-liquid buffer separation device 4, a self-balancing recovery device 7, and a separation chamber housing 8 , a separation chamber 16, a waste liquid recovery bottle 5 and a recovery three-way valve 6, the inlet of the buffer pump 2 is connected with the buffer liquid storage bottle 3 through a connecting pipe, and the upper end surface of the gas-liquid buffer separation device 4 has a gas-liquid buffer The separation device air inlet 17 and the gas-liquid buffer separation device liquid inlet 18, the lower end surface of the gas-liquid buffer separation device 4 has a plurality of gas-liquid buffer separation device liquid outlets 19; the separation chamber shell 8 is composed of a separation chamber upper cover plate 13, an ...

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PUM

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Abstract

The invention discloses an air exhausting device of a free-flow electrophoresis separation cavity and an implementation method of the air exhausting device, and belongs to the technical field of biochemical engineering. The air exhausting device comprises an air inflating pump, a hose clamp, a rubber hose, an air-liquid buffering and separation device, a self-balancing recycling device, a separation cavity shell, a separation cavity, a waste liquid recycling bottle and a recycling three-way valve. According to the air exhausting device, the liquid storage function of the air-liquid buffering and separation device is utilized, firstly, the buffering fluid which is over ten times as large as the volume of the separation cavity is stored in the air-liquid buffering and separation device, then the buffering fluid stored in the air-liquid buffering and separation device quickly enters into the separation cavity by virtue of a hand-operated air inflating pump, as the speed of the buffering fluid is high, the separation cavity can be filled with the buffering fluid soon, thus the air in the separation cavity is exhausted completely. The device disclosed by the invention can be used for exhausting the air in the separation cavity by utilizing very simple operations within a short period of time, so that the problem that the former separation cavity is cockamamie and difficult to exhaust air; and the device has the advantages of simple structure, low cost, practicability and effectiveness, and is easy to operate and the like.

Description

technical field [0001] The invention relates to the technical field of biochemical devices, in particular to a free-flow electrophoresis separation cavity exhaust device designed with an inflatable cylinder and an implementation method thereof. Background technique [0002] Free-flow electrophoresis technology is a liquid-phase preparative bioseparation technology that has been successfully applied to the separation of organelles, proteins, peptides, amino acids, etc., and has become an important separation technology in the fields of chemistry and biology. Since there is no solid medium, most of the water phase is used, so for free-flow electrophoresis, firstly, the recovery rate of the sample is almost 100%, and secondly, the mild water phase environment with good adaptability makes the enzyme or cells in the purification process Its biological activity is retained in the medium, and the whole electrophoresis is a continuous process, so free-flow electrophoresis can be use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D57/02
CPCG01N27/44704G01N30/0005G01N27/44769
Inventor 曹成喜颜健孔凡志杨成章樊柳荫
Owner SHANGHAI JIAO TONG UNIV
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