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Lindley eupatorium extract as well as preparation method and application thereof

An extract and wild horse technology, applied in the field of traditional Chinese medicine extracts, can solve the problems of poor color, increase production cost, waste energy, etc., and achieve the effect of high transfer rate and good activity

Active Publication Date: 2013-10-09
CHONGQING ACAD OF CHINESE MATERIA MEDICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. the separation and purification steps are single, and impurities cannot be effectively removed; 2. the purity of the product is not high, there are many impurities, and the color is not good; 3. organic reagents are used without recycling, causing serious environmental pollution problems ; ④Use a large amount of high-concentration ethanol, which increases production costs, wastes energy and is not environmentally friendly

Method used

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  • Lindley eupatorium extract as well as preparation method and application thereof
  • Lindley eupatorium extract as well as preparation method and application thereof
  • Lindley eupatorium extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The impact of different pretreatment modes of embodiment 1 on the transfer rate of total flavonoids

[0017] 1. Detection method of total flavonoids

[0018] Weigh Yemazhui ethanol extract, rutin reference substance, add appropriate amount of ethanol to dissolve, add 5%NaN / 2 1mL, place for 6min, then add 10%AL(N / 3 ) 3 1mL, stand for 6min, add 1m / l·L -1 Add 10mL of Na / H, add distilled water to make up to the mark, compare with the blank after 15min, and scan at the wavelength of 400-600nm by spectrophotometry. The results showed that the 70% ethanol extract of Yemazhui had the maximum absorbance at 490nm, and the reference substance of rutin had the maximum absorbance at 487nm, so 487nm was chosen to measure the absorbance of the sample.

[0019] Accurately measure 0.5, 1, 2, 3, 4, 5, and 6 mL of the rutin reference substance stock solution in a 25 mL volumetric flask, add distilled water to 10 mL, add 5% NaN / 2 1mL, place for 6min, then add 10%AL(N / 3 ) 3 1mL, ...

Embodiment 2

[0032] Example 2 Separation and purification of total flavonoids from wild horse

[0033] 1. Pretreatment before macroporous resin column chromatography

[0034] The 70% ethanol reflux group, alkaline pretreatment group and degreasing pretreatment group prepared in Example 1 were pumped into a rotary evaporator, concentrated until there was no ethanol smell, and the ethanol and concentrated clear paste were collected and recovered respectively. The reclaimed ethanol that collects can continue to be used in the extraction in the method in embodiment 1; For the concentrated clear cream that collects, according to clear cream (g): the ratio of the volume (mL) of pure water is the ratio of 1: 10~15, First add pure water to the clear paste, stir evenly, fully dissolve the clear paste, then pump the solution into the suction filter for suction filtration, and collect the filter residue and filtrate respectively. The collected filtrate is the upper column liquid; The collected filte...

Embodiment 3

[0042] Example 3 The active investigation of total flavonoid freeze-dried powder in the treatment of hyperlipidemia

[0043] 60 qualified mice were selected and randomly divided into 6 groups, 10 in each group. The Coptidis rhizome freeze-dried powder prepared in Example 2 was administered. 70% ethanol reflux group, alkaline pretreatment group and degreasing pretreatment group respectively according to crude drug 25.12, 25.56, 25.28g·kg -1 The dose of total flavonoids freeze-dried powder was fed; the atorvastatin calcium tablet group (positive control group) was 2.20mg·kg -1 Oral administration; the blank control group and the model group were each given equal volume of 1% hydroxymethylcellulose (CMC), 0.5mL·10g -1 , 1 time d -1 , 30 days in a row. 2 hours after the last drug administration, mice in each experimental group (except the blank control group) were intraperitoneally injected with 75% egg yolk milk, 0.5 mL per mouse, and 20 hours later, blood was collected from ...

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Abstract

The invention provides a lindley eupatorium extract and a preparation method thereof. Compared with the prior art, the lindley eupatorium extract is higher in transfer rate of total flavone, and meanwhile a total flavone offer has good activity for hyperlipidemia and liver cirrhosis diseases.

Description

technical field [0001] The present invention relates to a traditional Chinese medicine extract, and more specifically relates to a Ye Ma Zhuo extract, a preparation method and an application thereof. technical background [0002] Yemachai is the dry aboveground part of Eupat / rium Lindleyanum DC., a plant of Compositae. It tastes bitter and mild in nature. It has the effects of clearing lung and relieving cough, reducing phlegm and asthma, lowering blood pressure and blood lipid. The chemical components of Yemazhui whole grass are mainly flavonoids, as well as alkaloids, volatile oils, coumarins, and sesquiterpene esters. Flavonoids have obvious anti-ulcer, antispasmodic, anti-inflammatory and blood lipid-lowering series of biological activities. The preparations prepared by them are valuable in the treatment of cardiovascular and cerebrovascular diseases, arteriosclerosis, "three highs" and other diseases, and long-term use has no effect. toxic side effect. Current studies...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P9/10A61P1/16A61P3/06
Inventor 李卿
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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