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C. medinalis granulovirus bacillus thuringiensis bio-pesticide and preparation method thereof

A technology of rice leaf roller and granular virus, applied in the field of preparation of rice leaf roller granular virus Bacillus thuringiensis insecticide, rice leaf roller granular virus Bacillus thuringiensis biological insecticide, can solve the problem of persistent Short effective period and other issues, to achieve the effect of convenient operation, good product stability, and improved control effect

Active Publication Date: 2015-04-29
扬州绿源生物化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1980s, my country has carried out research on the control of rice pests with Bacillus thuringiensis biopesticides. A large number of tests have proved that Bacillus thuringiensis has a good control effect on rice leaf rollers and rice bract moths. up to 60.0%-76.4%, and also has a certain control effect on rice stem borer and rice stem borer, but the duration of effect is short

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A rice leaf roller granular virus Bacillus thuringiensis biological insecticide, the insecticide is made of the following raw materials in weight percentage: rice leaf roller granular virus 1.0×10 4 OB / mg, Bacillus thuringiensis 16000IU / mg, sodium lignosulfonate 3%, sodium methylene dinaphthalene sulfonate 3%, kaolin supplement 100%. Follow the steps below to prepare the biopesticide:

[0021] 1. Mix corn flour, bean cake powder, water and other raw materials evenly and add them to the fermenter, sterilize under high temperature and high pressure, then inoculate Bacillus thuringiensis strains, and culture them aseptically at 34°C until the strains produce spores and the spores fall off Afterwards, the culture was stopped, put into tanks, precipitated, and the supernatant was removed to obtain the Bacillus thuringiensis fermentation broth. Add surfactant sodium lignin sulfonate and sodium methylene dinaphthalene sulfonate in turn to Bacillus thuringiensis fermentation b...

Embodiment 2

[0027] A rice leaf roller granular virus Bacillus thuringiensis biological insecticide, the insecticide is made of the following raw materials in weight percentage: rice leaf roller granular virus 1.0×10 5 OB / mg, Bacillus thuringiensis 16000IU / mg, sodium lignosulfonate 3%, sodium methylene dinaphthalene sulfonate 3%, kaolin supplement 100%. Biopesticides were prepared according to the steps of Example 1.

Embodiment 3

[0029] A rice leaf roller granular virus Bacillus thuringiensis biological insecticide, the insecticide is made of the following raw materials in weight percentage: rice leaf roller granular virus 1.0×10 5 OB / mg, Bacillus thuringiensis 16000IU / mg, sodium lignosulfonate 5%, sodium methylene dinaphthalene sulfonate 5%, kaolin supplement 100%. Biopesticides were prepared according to the steps of Example 1.

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Abstract

The invention discloses a C. medinalis granulovirus bacillus thuringiensis bio-pesticide and a preparation method thereof. Main components of the bio-pesticide are C. medinalis granulovirus and bacillus thuringiensis, wherein the content of the C. medinalis granulovirus is 1.0*10<4>-1.0*10<8>OB / mg, and the titer of bacillus thuringiensis is 16000IU / mg. The bio-pesticide is prepared by using bio-pesticide, bacillus thuringiensis, lignin sodium sulfonate, methylene naphthalene sulfonate, and kaolin according to a certain ratio. The bio-pesticide provided by the invention has the advantages of wide insecticidal spectrum, fast effect, good product stability, safety to environment, human and livestock, and no residue. The method has the advantages of simple process and convenient operation. With the bio-pesticide, occurrence and harm of C. medinalis can be effectively controlled. The bio-pesticide can be applied with large amount on paddy rice, especially on pollution-free rice and organic rice.

Description

technical field [0001] The invention relates to a microbial insecticide, in particular to a rice leaf roller granular virus bacillus thuringiensis biological insecticide, which is suitable for preventing and controlling rice leaf rollers, rice stem borers, rice bract moths and other Lepidoptera pests. The invention also relates to a method for preparing the rice leaf roller granular virus bacillus thuringiensis insecticide. Background technique [0002] Rice leaf roller ( Cnaphalocrocis medinalis Guenee) is an important migratory pest of rice. The larvae roll the leaves vertically to eat and cause damage, leaving only the epidermis and forming white streaks, which affect the photosynthesis of crops. In the middle and late stages of rice, it will damage the upper functional leaves and affect the filling, resulting in unfulfilled seeds, increased hollow rate, and reduced thousand-grain weight. Generally, the yield can be reduced by 20-30%, and the weight is more than 50%. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P7/04
Inventor 刘琴徐健李传明祁建杭韩光杰王艳蔡扬生
Owner 扬州绿源生物化工有限公司
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