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31results about How to "Shorten the time to death" patented technology

Ship ballast water treating method

InactiveCN104016533AAvoid the disadvantage of low lifespanEnhanced inactivationMultistage water/sewage treatmentOzone generatorUltrasonic sensor
The invention discloses a ship ballast water treating method which comprises the following steps: (1), filtering seawater by a filter; (2), carrying out ozone oxidization and sterilization; (3), carrying out ultrasonic-wave cavitation and sterilization; (4), carrying out ultraviolet sterilization; (5), conveying the water into a ballast bin as ballast water; (6), carrying out back-flushing with seawater for a filter after a ballasting process is ended; (7), treating according to the steps (2), (3) and (4) before the ballast water is exhausted; (8), exhausting the treated and qualified ballast water. According to the invention, synergistic effects of ozone oxidization and sterilization, ultrasonic-wave cavitation and sterilization and ultraviolet-light radiation sterilization are performed, equipment is small in size, low in energy consumption, high in treating efficiency and wide in range of application. Moreover, treatment scale can be flexibly regulated by increasing or reducing number of ozone generators, ultrasonic transducers, low-pressure mercury lamps, so that the treatment capacity can be 20m<3> / h-5000m<3> / h. Besides, organic pollutants in seawater can be degraded while microorganisms are killed, so that the water quality of the exhausted ballast water is improved.
Owner:SHANGHAI JIAO TONG UNIV +1

Recombinant fungus targeting expression of silent pest-pattern recognition protein GNBP3 gene and application of recombinant fungus in pest control

ActiveCN110759983AIncreased mortalityTime to death shortenedBiocideFungiBacilliVermin
The invention discloses a recombinant fungus targeting expression of silent pest-pattern recognition protein GNBP3 gene and application of the recombinant fungus in pest control. The invention constructs recombinant beauveria bassiana capable of inhibiting or blocking GNBP3 gene transcription. Therefore, when the recombinant beauveria bassiana infects pests, the expression of antibacterial peptidein the pests is remarkably reduced, the signal transduction of pest recognition immune entomogenous fungi is weakened or interrupted, and the pests are more easily infected with pathogenic microorganisms such as the entomogenous fungi to die. The recombinant beauveria bassiana significantly improves the death rate of the pests, shortens the death time, and has concentration dependence. The recombinant beauveria bassiana is combined with a microbial insecticide bacillus thuringiensis Bt or serratia marcescens so as to significantly improve the death rate and death time of pests, it is indicated that the recombinant beauveria bassiana is a very good synergist of the microbial insecticide, does not improve the killing effect, widens the insecticidal spectrum, reduces the drug resistance of the microbial insecticide, and is an environment-friendly microbial insecticide.
Owner:SOUTH CHINA AGRI UNIV

Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)

InactiveCN103814950AInsecticide works quicklyTime to death shortenedBiocideAnimal repellantsInclusion bodiesLectin
The invention belongs to the technical field of biological Helicoverpa armigera prevention and control, and particularly relates to a method for improving the biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs). The method can improve the Helicoverpa armigera prevention and control effect of NPVs through the feeding of a Helicoverpa armigera C-type lectin dsRNA (double-stranded ribonucleic acid) (Ha-lectin1 dsRNA); the Helicoverpa armigera prevention and control effect of NPVs can be remarkably improved at a dosage of 3 micrograms of Ha-lectin1 dsRNA and 50 PIBs (polyhedral inclusion bodies) of NPVs per larva of Helicoverpa armigera. The method provided by the invention has a fast onset of insect killing, that is, the time of Helicoverpa armigera death caused by Helicoverpa armigera NPVs (HaNPVs) can be shortened by 1 / 3; the insect killing efficiency is high, that is, the killing rate in the first three days can be increased by 35 percent; the consumption is low, that is, the use consumption of NPVs can be greatly reduced. Compared with the method which simply uses NPVs for Helicoverpa armigera prevention and control in the prior art, the method provided by the invention achieves remarkable improvements.
Owner:HENAN UNIVERSITY

Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)

The invention belongs to the technical field of biological Helicoverpa armigera prevention and control, and particularly relates to a method for improving the biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs). The method can improve the Helicoverpa armigera prevention and control effect of NPVs through the feeding of a Helicoverpa armigera C-type lectin dsRNA (double-stranded ribonucleic acid) (Ha-lectin1 dsRNA); the Helicoverpa armigera prevention and control effect of NPVs can be remarkably improved at a dosage of 3 micrograms of Ha-lectin1 dsRNA and 50 PIBs (polyhedral inclusion bodies) of NPVs per larva of Helicoverpa armigera. The method provided by the invention has a fast onset of insect killing, that is, the time of Helicoverpa armigera death caused by Helicoverpa armigera NPVs (HaNPVs) can be shortened by 1 / 3; the insect killing efficiency is high, that is, the killing rate in the first three days can be increased by 35 percent; the consumption is low, that is, the use consumption of NPVs can be greatly reduced. Compared with the method which simply uses NPVs for Helicoverpa armigera prevention and control in the prior art, the method provided by the invention achieves remarkable improvements.
Owner:HENAN UNIVERSITY

A recombinant fungus targeting the expression of the gene expression of the silencing pest pattern recognition protein gnbp3 and its application in pest control

The invention discloses a recombinant fungus targeted at silencing the expression of the pest pattern recognition protein GNBP3 gene and its application in pest control. The invention constructs a recombinant Beauveria bassiana that can inhibit or block the transcription of the GNBP3 gene. As a result, when the recombinant Beauveria bassiana infects pests, the expression of antimicrobial peptides in the pests is significantly down-regulated, weakening or interrupting the signal transduction of pests to recognize and immune fungi, and making pests more susceptible to infection and death by pathogenic microorganisms such as fungi. Recombinant Beauveria bassiana significantly increased the mortality of pests and shortened the death time in a concentration-dependent manner. Combining it with microbial-derived insecticides Bacillus thuringiensis Bt or Serratia marcescens can significantly increase the mortality and death time of recombinant Beauveria bassiana against pests, indicating that it is a good microbial insecticide. The synergist, not to mention improving the killing effect, broadens the insecticidal spectrum, reduces the resistance of microbial insecticides, and is an environmentally friendly microbial source insecticide.
Owner:SOUTH CHINA AGRI UNIV

Composite insect virus synergist and preparation method thereof

The invention discloses a composite insect virus synergist, which comprises the following components in percentage by volume: 2 to 8 percent of aqueous solution of toxin protein, 0.3 to 1.5 percent of emulsifiable solution of cantharidin, 1 to 3 percent of Tween-80 emulsifier and the balance of 1*PBS buffer solution, wherein the total volume of the components is 100 percent. The preparation method of the synergist is implemented by the following steps: constructing a toxin protein expressing genetic engineering strain; inducing the expression of the toxin protein; collecting expressed toxin protein; preparing aqueous solution of active toxin protein; mixing the components to obtain the composite insect virus synergist. The composite insect virus synergist and the preparation method thereof have the advantages that: the preparation process is simple; the prepared synergist can improve the insecticidal activity of insect virus insecticides and improve control efficiency.
Owner:NORTHWEST A & F UNIV

Negative-pressure harmless method for rapidly and efficiently treating fruits damaged by boring pests

The invention relates to a negative-pressure harmless method for rapidly and efficiently treating fruits damaged by boring pests. The method is carried out by adopting a negative-pressure harmless treatment device. The method comprises the following steps: 1) collecting damaged fruits and vegetables, putting the damaged fruits and vegetables into a treatment bag, and sealing the treatment bag through a sealing strip; (2) starting a mobile power source and a negative-pressure air extractor, conducting air extraction on the treatment bag of the insect fruit treatment part through the negative-pressure air extractor till an airflow meter displays zero, after air extraction is completed, connection between an air valve sealing opening and an air valve butt-joint opening is switched off, and rapid and efficient harmless treatment on the fruits damaged by the boring pests is achieved. The method is easy to operate, environmentally friendly, low in cost and high in effect, solves the problems that in the prior art, the fruits damaged by the boring pests in the field are not thoroughly treated, the lethal speed is low, and the effect is poor, shows the characteristics of thoroughness, fast-acting property, high efficiency and cheapness of damaged fruit treatment, and can rapidly and efficiently conduct negative-pressure harmless treatment on the fruits damaged by the boring pests.
Owner:SHANDONG INST OF POMOLOGY
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