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Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)

A nuclear polyhedrosis, cotton bollworm technology, applied in the fields of botanical equipment and methods, insecticides, biocides, etc., can solve the problems of slow insecticidal effect, hinder the popularization and use, long effective period, etc., and achieve effective insecticidal effect. Faster action, reduced risk of resistance, reduced harm

Inactive Publication Date: 2014-05-28
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton bollworm nuclear polyhedrosis virus (Helicoverpa armigera NPV, HaNPV) specifically parasitizes cotton bollworm, but because of its slow insecticidal effect and long effective period, it is different from the "drug" required by farmers. There is a large gap between "to insect death", which seriously hinders its popularization and use

Method used

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  • Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)
  • Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)
  • Method for improving biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs)

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Embodiment 1

[0022] On the basis of work practice and research on prior art literature, the inventor found that C-type lectin (Ha-lectin1) in cotton bollworm can inhibit the proliferation of HaNPV in cotton bollworm , the inventors have carried out a series of experiments to prove this fact.

[0023] (1) RNA interference experiment

[0024] According to the cloned C-type lectin gene sequence of cotton bollworm (NCBI gene ID: ABF83203), we selected the 417bp region with low similarity with other sequences of cotton bollworm as the target sequence, and designed RNAi primers.

[0025] The above-mentioned 417bp cotton bollworm gene cDNA sequence has been published in the existing literature, and for the convenience of those skilled in the art to implement the present invention, it is republished as follows:

[0026] 5’-tacaagttccacagagtaccaggaacttttgatcgggctcactttgtttgttcggctgaaaacggccatcttgccattataaatagtgaagatgaagcggaagttttgagaaaagtctttgctgataatcctgctgcatggatacctggaaatttttggaaagacatagc...

Embodiment 2

[0046] Through a series of experiments in Example 1, it has been fully proved that C-type lectin (Ha-lectin1) can inhibit the proliferation of HaNPV in the cotton bollworm, so by inhibiting the transcription of the C-type lectin (Ha-lectin1) gene and expression, and silencing the C-type lectin (Ha-lectin1) gene of the cotton bollworm can enhance the insecticidal effect of HaNPV.

[0047] Studies have found that feeding dsRNA in insects can also cause silencing of target genes. Therefore, the inventors conducted further research on the feeding dosage of dsRNA of C-type lectin (Ha-lectin1) in cotton bollworm. The specific experimental research is as follows:

[0048] (1) Gene silencing caused by feeding different concentrations of dsRNA

[0049] Select 6 instar 12h cotton bollworm larvae, 20 in each group, and divide them into 4 groups. After 12 hours of starvation treatment, feed and spray 1ul concentrations of 0.5 ug / ul, 2.5 ug / ul, 3.0 ug / ul and 5.0 ug / ul respectively Ha-le...

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Abstract

The invention belongs to the technical field of biological Helicoverpa armigera prevention and control, and particularly relates to a method for improving the biological Helicoverpa armigera prevention and control effect of nuclear polyhedrosis viruses (NPVs). The method can improve the Helicoverpa armigera prevention and control effect of NPVs through the feeding of a Helicoverpa armigera C-type lectin dsRNA (double-stranded ribonucleic acid) (Ha-lectin1 dsRNA); the Helicoverpa armigera prevention and control effect of NPVs can be remarkably improved at a dosage of 3 micrograms of Ha-lectin1 dsRNA and 50 PIBs (polyhedral inclusion bodies) of NPVs per larva of Helicoverpa armigera. The method provided by the invention has a fast onset of insect killing, that is, the time of Helicoverpa armigera death caused by Helicoverpa armigera NPVs (HaNPVs) can be shortened by 1 / 3; the insect killing efficiency is high, that is, the killing rate in the first three days can be increased by 35 percent; the consumption is low, that is, the use consumption of NPVs can be greatly reduced. Compared with the method which simply uses NPVs for Helicoverpa armigera prevention and control in the prior art, the method provided by the invention achieves remarkable improvements.

Description

technical field [0001] The invention belongs to the technical field of biological control of cotton bollworm, and in particular relates to a method for enhancing the effect of nuclear polyhedrosis virus biological control of cotton bollworm. Background technique [0002] Cotton bollworm is a worldwide omnivorous pest, which not only harms cotton, but also harms various crops such as corn and wheat and vegetables such as tomato and pepper. In recent years, with the cultivation of transgenic cotton varieties, it has played a huge role in controlling cotton bollworm pests. However, with the long-term and large-scale planting of BT transgenic cotton, cotton bollworms have begun to develop resistance in the field, so the control situation of cotton bollworms is still very serious. urgent. [0003] Nuclear polyhedrosis viruses (nuclear polyhedrosis viruses, NPV) are a type of virus with a strong specific host. Because of its high safety, long duration and environmental friendline...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N57/16A01P7/04C12N15/113C12N15/10
Inventor 柴连琴孟景会王树芳王楠周文杰
Owner HENAN UNIVERSITY
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