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A kind of pesticide for controlling cotton bollworm by nuclear polyhedrosis virus and its preparation method and application

A technology of nuclear polyhedron and cotton bollworm, applied in the direction of botanical equipment and methods, applications, pesticides, etc., can solve the problem of no control

Active Publication Date: 2021-08-20
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nuclear polyhedrosis virus is the earliest commercial virus biopesticide, and it is an important pathogenic microorganism of lepidopteran insects. The feces and dead insects of diseased insects can infect other larvae, and can also be passed on to offspring through eggs, but it is suitable for children under the third instar The young larvae have almost no control effect on the mature cotton bollworm above the 3rd instar, especially the 6th instar

Method used

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  • A kind of pesticide for controlling cotton bollworm by nuclear polyhedrosis virus and its preparation method and application
  • A kind of pesticide for controlling cotton bollworm by nuclear polyhedrosis virus and its preparation method and application
  • A kind of pesticide for controlling cotton bollworm by nuclear polyhedrosis virus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A pesticide for controlling nuclear polyhedrosis virus against cotton bollworm, the active ingredients of which are nuclear polyhedrosis virus and dew grass extract containing ecdysterone. The two are mixed according to a certain ratio.

[0026] In order to identify the effects of different proportions and concentrations of nuclear polyhedrosis virus and dew grass extract on the 6th instar cotton bollworm, experiments were carried out according to Table 1 to determine the survival rate. Divided into 12 groups, 50 heads in each group. The results are shown in Table 1. Wherein the preferred embodiment provided by the present invention, that is, the experimental method of the pesticide with one of the concentrations is as follows, and the experimental methods of other groups are similar and can be deduced according to those skilled in the art.

[0027] Dissolve 21 grams of dew grass extract containing 30% ecdysone (purchased from Xi'an Ruiying Biotechnology Co., Ltd.) wi...

Embodiment 2

[0047] A preparation method of a pesticide for controlling cotton bollworm by nuclear polyhedrosis virus, specifically comprising the steps of:

[0048] (1) Dissolve the dew grass extract containing 1.6-2.0Kg ecdysterone with water, and mix well to obtain the dew grass extract solution;

[0049] (2), the dew grass extract solution and containing 2.8 × 10 12 PIB-3.5×10 12 Solutions of PIB nuclear polyhedrosis virus were mixed to obtain the pesticide.

[0050] Application of a nuclear polyhedrosis virus pesticide in biological control of cotton bollworm. The application method per mu is as follows: the prepared nuclear polyhedrosis virus pesticide will be diluted to contain 1.6-2.0Kg ecdysone and 2.8×10 12 PIB-3.5×10 12 PIB nuclear polyhedrosis virus pesticide preparation is 500-1000mL, diluted to a certain volume, such as 20-30Kg, mixed and sprayed.

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Abstract

The invention belongs to the technical field of biological control of cotton bollworm, and the effect of preventing and controlling mature cotton bollworm can be greatly improved by adding dew grass extract containing ecdysterone to the nuclear polyhedrosis virus of cotton bollworm. it is good. According to each 6th instar cotton bollworm 5 mg dew grass extract (30% ecdysone) and 2.0×10 6 The dosage of PIB nuclear polyhedrosis virus can significantly enhance the effect of nuclear polyhedrosis virus on controlling mature cotton bollworm. The pesticide provided by the present invention has a mortality rate of 100% within 48 hours for larvae under the third instar; it has fast onset of action, high efficiency, and reduced egg production for the prevention and control of mature cotton bollworms, and has a significant effect on the prevention and control of the outbreak of cotton bollworms in the next year. It fills the gap that there is no effective killing of cotton bollworm at present.

Description

technical field [0001] The invention belongs to the technical field of biological control of cotton bollworm, and in particular relates to a pesticide for controlling cotton bollworm by nuclear polyhedrosis virus, a preparation method and application thereof. Background technique [0002] Cotton bollworm is a serious omnivorous pest. The mature 5-6 instar larvae of cotton bollworm enter the gluttony stage, which is the most harmful to crops. Cotton bollworm is also the species that lays the most eggs among insects. A single female moth can lay more than 3,000 eggs at most. If the control is not in place, a single overwintering female pupa can reproduce tens of millions of larvae in the coming year on average, and if there are too many eggs, it will grow up. Therefore, a small number of mature cotton bollworms that slip through the net will have a devastating effect. But at present, both chemical pesticides and biological pesticides are less effective in controlling mature c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/60A01N45/00A01P7/04
CPCA01N45/00A01N63/60
Inventor 柴连琴荆玉谱张林杰刘鹏飞孟景会张海燕王喆
Owner HENAN UNIVERSITY
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