Virus insecticidal synergist and its preparation method and application in virus insecticide

A virus insecticide and virus insecticide technology, applied in the field of genetic engineering, can solve the problems of high cost, in-depth research on the function of ENHANCIN, and poor effect

Inactive Publication Date: 2008-07-02
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It is worth noting that although ENHANCIN has been applied to the research of recombinant viruses and transgenic plants, the real application method that can be implemented is to apply the enhanced protein prokaryotic expression and then mix it with virus preparations, or mix GV directly with NPV, High cost and poor effect
The reason for the existence of these problems can be attributed to the fact that the current research on the function of ENHANCIN is still not in-depth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: First prepare the virus insecticidal synergist: directly inoculate Escherichia coli transformed with a recombinant plasmid containing the ENHANCIN active fragment in sterile LB culture medium, expand the culture to saturation at 37°C, OD≈4, and collect Bacteria can be.

[0012] Then prepare the virus insecticide: prepare the following raw materials in parts by volume: chlorfluazuron 0.01~0.05, tryptophan 0.01~0.1, dispersant 1~2, glycerin 2~5, fluorescent whitening agent 0.2~0.5, sterile water 92.35-96.78, after stirring them evenly, add baculovirus into it at a ratio of 5-2 billion PIB / ml, and finally add virus insecticidal synergist at a ratio of 2-4 billion / ml, and stir evenly. The dispersant can be any one of L602, LH656 or L656.

Embodiment 2

[0013] Embodiment 2: In this embodiment, the same method as in Embodiment 1 is used to prepare a virus and insecticide synergist.

[0014] Select 0.1 ml of chlorfluazuron, 0.3 ml of tryptophan, 10 ml of L656 dispersant, 20 ml of glycerin, 5 ml of fluorescent whitening agent, 2,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000, and 2, 000,000,000,000,000,000. . First, mix the granule virus, chlorfluazuron, and virus insecticidal synergist, then add glycerin, tryptophan, dispersant, and fluorescent whitening agent in sequence, and finally add sterile water to make up to 1 liter, and stir Evenly, to obtain a suspending agent.

Embodiment 3

[0015] Embodiment 3: In this embodiment, the virus insecticide synergist is prepared with the same method as in Embodiment 1.

[0016] Select 0.5 ml of chlorfluazuron, 1 ml of tryptophan, 10 ml of LH656 dispersant, 20 ml of glycerin, 2 ml of fluorescent whitening agent, 500 billion PIBs of P. . First, mix the granule virus, chlorfluazuron, and virus insecticidal synergist, then add glycerin, tryptophan, dispersant, and fluorescent whitening agent in sequence, and finally add sterile water to make up to 1 liter, and stir Evenly, to obtain a suspending agent.

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Abstract

The invention discloses a virus desinsection synergistic agent which relates to a prokaryotic expression bacterium liquid of an active central section of an ENAHANCIN increasing protein. The active central section relates to the amino acids from 22 to 380 at an N end of the ENAHANCIN protein and the molecular weight is 33000 daltons. The invention also provides a preparing method for the virus desinsection synergistic agent and an application method of the virus desinsection synergistic agent in virus pesticide. The virus desinsection synergistic agent can greatly improve the pathogenicity of the virus, has remarkable increasing effect on the virus pesticide and has good application value.

Description

technical field [0001] The invention belongs to genetic engineering, in particular to a virus insecticide synergist. Background technique [0002] In recent years, chemical pesticides have played a huge role in increasing agricultural production and harvest, but they have also caused some specific problems. On the one hand, agricultural and forestry pests have developed strong resistance, and on the other hand, they have seriously polluted the natural environment. Therefore, the use of biological pesticides The development and application have been paid more and more attention at home and abroad. Baculovirus insecticide is an important representative of biological pesticides. It has many advantages such as good insecticidal effect, no pollution to the environment, no harm to natural enemies, and no harm to humans and other higher animals, so it has broad application prospects. However, baculovirus insecticides have the outstanding defect of slow insecticidal speed, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N1/21A01P7/04C12R1/19
Inventor 张小霞梁振普
Owner HENAN AGRICULTURAL UNIVERSITY
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