Application of melatonin in delaying paddy leaf senescence and tolerating salt

A technology of melatonin and leaves, which is applied in the application field of melatonin in delaying rice leaf senescence and salt tolerance, and can solve the problems that genes cannot be applied, complex genetic characteristics of transgenic plants, etc.

Inactive Publication Date: 2015-07-15
百格基因科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, premature senescence of leaves has extremely adverse effects on the formation of yield traits, especially in hybrid rice varieties with strong yield advantages, and premature senescence has seriously threatened the full development of yield potential
At present, several key senescence regulatory genes (such as OsNAP, OsDOS, OsTZF1, etc.) have been cloned in rice. However, due to the complex genetic characteristics of transgenic plants and extensive social controversy, these genes have not yet been applied in actual agricultural production.

Method used

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  • Application of melatonin in delaying paddy leaf senescence and tolerating salt
  • Application of melatonin in delaying paddy leaf senescence and tolerating salt
  • Application of melatonin in delaying paddy leaf senescence and tolerating salt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Melatonin plays a key role in delaying rice leaf senescence and cell death

[0036] 1. Melatonin Treatment Concentration Screening

[0037] Take Nipponbare seedlings that have germinated for 2 weeks, and culture them with nutrient solutions containing different concentrations of melatonin for 10 days (16 hours of light / 8 hours of darkness per day), then take pictures, observe the growth of the plants, and measure the length of the aboveground parts of the plants. The concentrations of melatonin were 10 μM, 20 μM, 50 μM, 100 μM, 500 μM, and 1000 μM, and a control treatment without adding melatonin (ie, the concentration of melatonin was 0 μM) was set. Three replicates were performed, with 10 plants treated for each melatonin concentration in each replicate.

[0038] see photos figure 1 A, the length of the above-ground part of the plant see figure 1 b. Low concentration of melatonin (10μM and 20μM) can obviously promote the growth of seedlings. When it wa...

Embodiment 2

[0048] Example 2, Melatonin Improves Salt Resistance of Rice and Delays Salt-Induced Leaf Senescence and Cell Death

[0049] Salt treatment: Nipponbare seedlings germinated for 2 weeks were taken and cultured in a nutrient solution containing melatonin (10 μM or 20 μM) and 0.5 g / 100 ml sodium chloride for 14 days (16 hours of light / 8 hours of darkness per day) (set without adding melatonin control treatment of melanin), and then the plants were photographed, the leaves were taken for trypan blue staining, and then the leaves were taken to detect the chlorophyll content in the leaves.

[0050] Normal treatment: Nipponbare seedlings germinated for 2 weeks were taken and cultured for 14 days in a nutrient solution containing 10 μM or 20 μM melatonin (16 hours of light / 8 hours of darkness per day) (a control treatment without adding melatonin was set), and then the plants were Take pictures, take the leaves for trypan blue staining, take pictures, take the leaves and detect the ch...

Embodiment 3

[0053] Embodiment 3, melatonin reduces intracellular H 2 o 2 Horizontal delay of leaf senescence and cell death

[0054] 1. Analysis of RNA-seq sequencing results

[0055] RNA-seq transcriptome analysis showed that compared with Nipponbare treated with 20 μM melatonin and Nipponbare without melatonin, a total of 457 differentially expressed genes were obtained, including 191 up-regulated genes and 266 down-regulated genes . Cluster analysis of these genes revealed that the most significantly enriched gene category among differentially expressed genes was redox-related genes, including 28 up-regulated and 21 down-regulated antioxidant genes. This result suggests that melatonin may have an important biological function in the process of hydrogen peroxide scavenging.

[0056] 2. Exogenous application of melatonin effectively reduces the content of hydrogen peroxide under dark and salt treatment

[0057] Dark treatment: Nipponbare seedlings germinated for 2 weeks were taken and...

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Abstract

The invention discloses application of melatonin in delaying paddy leaf senescence and tolerating salt. The invention sets forth application of melatonin in delaying plant senescence. The plant senescence specifically can be salt-induced plant senescence and / or dark-induced plant senescence and / or age-mediated plant senescence. The plant senescence specifically can be embodied by (a) and / or (b) and / or (c) and / or (d) and / or (e) below: (a) plant senescence; (b) leaf senescence; (c) cell death; (d) leaf cell death; and (e) leaf photosynthetic rate decline. The application is helpful to deeply understanding a melatonin-mediated senescence control signal network, meanwhile, provides effective reference and help for prevention and control of senescence in agriculture, horticulture and flowers, and has potential application value in high-yield molecular breeding and preservation and transportation of harvested vegetables and flowers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of melatonin in delaying rice leaf senescence and salt tolerance. Background technique [0002] Melatonin (also known as melatonin, melatonin, epinenaline), whose chemical formula is N-acetyl-5-methoxytryptamine, is present in many organisms from algae to humans An amine hormone. Melatonin presents circadian rhythm changes in organisms, and with age, its content level gradually decreases, and the circadian rhythm gradually becomes flat or even disappears. Melatonin was discovered and reported for the first time in plants in 1995, and subsequent studies have shown that melatonin also widely exists in microtubule plants. [0003] Aging is a programmed death process after the completion of mitosis, which is precisely regulated by endogenous genetic material. Since senescence initiation and senescence rate have important effects on the time of plant effecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 梁成真钱扬文储成才
Owner 百格基因科技(江苏)有限公司
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