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Kit for detecting relative risk gene of liver cancer

A kit and gene technology, applied in the fields of molecular biology and medicine, can solve problems such as unmethylation of CⅡTA gene

Inactive Publication Date: 2013-10-23
浙江爱易生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various cytokines such as IFN-γ can induce the expression of HLA class Ⅱ molecules in blood-derived or non-blood-derived tissues, but the CⅡTA gene must be unmethylated in advance

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1. Use of detection kits

[0030] Step 1: Extraction of DNA template

[0031] Vacuum blood collection tubes are used to collect human peripheral blood, and the genomic DNA in the blood is extracted.

[0032] Step 2: Real-time quantitative PCR reaction

[0033] Use a fluorescent quantitative PCR detection kit that can detect liver cancer-related risk genes, which includes the following primer pairs and fluorescent probe pairs:

[0034] Sense Primer 1: 5'- TTTTAATGACAACA - 3' (SEQ ID NO: 1)

[0035] Antisense Primer 1: 5'- ATCGTCTCCCCAAAG - 3' (SEQ ID NO: 2)

[0036] Sense Primer 2: 5'- ATTATGGTGATTCT - 3' (SEQ ID NO: 3)

[0037] Antisense Primer 2: 5'-ACTTAGACTAGGTC-3' (SEQ ID NO: 4)

[0038] Probe 1 with VIC fluorophore: 5'-TTTTATcGTGTAG-3' (SEQ ID NO: 5)

[0039] Probe 1 with FAM fluorophore: 5'-TTTTATtGTGTAG-3' (SEQ ID NO: 6)

[0040] Probe 2 with VIC fluorophore: 5'-ATAGCAaCTTCAT-3' (SEQ ID NO: 7)

[0041] Probe 2 with FAM fluorophore: 5'-ATAGCAgCTTCA...

Embodiment 2

[0049] Example 2. Services for liver cancer-related risk gene testing for patients

[0050] Step 1: DNA Extraction

[0051] The laboratory physicians of the hospital collect peripheral blood from the subjects, use vacuum blood collection tubes to collect peripheral blood, and extract genomic DNA from it.

[0052] Step 2: Genotyping Assays

[0053] Using the kit provided by the present invention, the SNP site No. rs2596542 on the MICA gene of the subject's genomic DNA and the SNP site No. rs9275572 on the HLA-DQ gene are respectively detected by fluorescent quantitative PCR to determine the two SNPs sites genotype.

[0054] Step 3: Analysis of individual risk of liver cancer

[0055] Through the analysis of the SNPs genotype of the tested subjects, an analysis report of the risk of suffering from liver cancer is issued. The report details the risk of the subject suffering from liver cancer, and the doctor explains and interprets the analysis report sheet of the risk of suff...

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Abstract

The invention discloses a kit for detecting relative risk gene of liver cancer. The kit comprises a specific primer pair and a specific fluorescence probe pair for simultaneously detecting an rs2596542# SNP locus on an MICA gene and an rs9275572# SNP locus on an HLA-DQ gene and a conventional component for fluorogenic quantitative PCR detection. According to the kit, the risk of suffering liver cancer is evaluated by simultaneously detecting the single nucleotide polymorphism locus gene type of MICA and HLA-DQ closely related to liver cancer.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting risk genes associated with liver cancer, by simultaneously detecting single nucleotide polymorphisms of genes MICA and HLA-DQ associated with liver cancer Point genotypes were used to assess individual risk of liver cancer. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People often say that liver cancer refers to primary liver cancer. Primary liver cancer is one of the most common malignant tumors in clinical practice. According to the latest statistics, there are about 600,000 new liver cancer patients in the world every year, ranking fifth among malignant tumors. With the continuous in-depth study of human genes, two risk genes related to liver cancer have been discovered, namely MICA and HLA-D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 任峻张华忠
Owner 浙江爱易生物医学科技有限公司
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