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PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog

A detection kit and technology for hydatid disease are applied in the fields of PCR primers, diagnosis of animal infectious diseases, and general-purpose PCR kits, which can solve problems such as reports and no detection kits.

Inactive Publication Date: 2013-10-30
WITHYOU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been reports on the PCR detection method for canine cystic echinococcosis at present, there is no report on the relevant detection kit

Method used

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  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog
  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog
  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take a dog suspected of suffering from echinococcosis, scrape a little feces, and operate according to the procedure of the kit. The results are shown in the attached figure 1 shown. According to the results, it was judged that the dog was infected with echinococcosis. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 2

[0027] Take a dog suspected of suffering from echinococcosis, scrape a little feces, and operate according to the procedure of the kit. The results are shown in the attached figure 2 shown. According to the results, it was judged that the dog was infected with echinococcosis. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 3

[0030]

[0031]

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PUM

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Abstract

The invention discloses a PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of a dog. According to the method, a pair of primers is designed according to a 12S rDNA (ribosome Deoxyribose Nucleic Acid) sequence conserved region of pathogene echinococcus granulosus of the cystic echinococcosis, a PCR reaction is carried out with the DNA contained in the wastes of an animal to be detected serving as a template, and then a comparison is carried out relative to an attached negative control and a positive control, in order to determine whether the detected animal infects the echinococcosis. The detection kit is simple and easy to use, and high in sensitivity and stability and can be used for monitoring the health condition of a pet dog.

Description

technical field [0001] The invention belongs to the diagnosis technology of animal infectious diseases, and relates to a detection method for canine cystic echinococcosis. [0002] The invention provides a universal PCR kit for detecting canine cystic echinococcosis. The content of the present invention relates to a pair of PCR primers used to detect canine cystic echinococcosis, and a set of PCR detection comprising PCR reaction mix (including primers), positive control, negative control, and bromophenol blue loading buffer Kits and corresponding detection procedures. Background technique [0003] Echinococcosis, also known as hydatid disease, is a serious disease caused by echinococcus or hydatidcyst, the larvae of Echinococcus tapeworms, parasitic in animals (including humans). Zoonotic parasitic diseases that endanger human health are distributed all over the world. The genus Echinococcus (tapeworm) is taxonomically belonging to the Phylum Platyhelminthes, Order Round...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 朱威贺雄雷潘岷溟薛虎平郭蕾
Owner WITHYOU BIOTECH
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