PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog

A detection kit and technology for hydatid disease are applied in the fields of PCR primers, diagnosis of animal infectious diseases, and general-purpose PCR kits, which can solve problems such as reports and no detection kits.

Inactive Publication Date: 2013-10-30
WITHYOU BIOTECH
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been reports on the PCR detection method for canine cysti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog
  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog
  • PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of dog

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take a dog suspected of suffering from echinococcosis, scrape a little feces, and operate according to the procedure of the kit. The results are shown in the attached figure 1 shown. According to the results, it was judged that the dog was infected with echinococcosis. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 2

[0027] Take a dog suspected of suffering from echinococcosis, scrape a little feces, and operate according to the procedure of the kit. The results are shown in the attached figure 2 shown. According to the results, it was judged that the dog was infected with echinococcosis. The test paper test result was also positive, confirming that the PCR test result was correct.

Embodiment 3

[0030]

[0031]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) detection kit for cystic echinococcosis of a dog. According to the method, a pair of primers is designed according to a 12S rDNA (ribosome Deoxyribose Nucleic Acid) sequence conserved region of pathogene echinococcus granulosus of the cystic echinococcosis, a PCR reaction is carried out with the DNA contained in the wastes of an animal to be detected serving as a template, and then a comparison is carried out relative to an attached negative control and a positive control, in order to determine whether the detected animal infects the echinococcosis. The detection kit is simple and easy to use, and high in sensitivity and stability and can be used for monitoring the health condition of a pet dog.

Description

technical field [0001] The invention belongs to the diagnosis technology of animal infectious diseases, and relates to a detection method for canine cystic echinococcosis. [0002] The invention provides a universal PCR kit for detecting canine cystic echinococcosis. The content of the present invention relates to a pair of PCR primers used to detect canine cystic echinococcosis, and a set of PCR detection comprising PCR reaction mix (including primers), positive control, negative control, and bromophenol blue loading buffer Kits and corresponding detection procedures. Background technique [0003] Echinococcosis, also known as hydatid disease, is a serious disease caused by echinococcus or hydatidcyst, the larvae of Echinococcus tapeworms, parasitic in animals (including humans). Zoonotic parasitic diseases that endanger human health are distributed all over the world. The genus Echinococcus (tapeworm) is taxonomically belonging to the Phylum Platyhelminthes, Order Round...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
Inventor 朱威贺雄雷潘岷溟薛虎平郭蕾
Owner WITHYOU BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap