CD3<+>CD56<+>T cell for FOXP3 expression and application thereof
A technology of cells and lymphocytes, applied in the application field of prognosis information of patients with liver cancer, can solve the problems of undiscovered research reports and other issues
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Embodiment 1
[0198] Example 1. Local infiltration of CD3 in hepatocellular carcinoma tissue and paracancerous tissue + CD56 + Changes in the proportion of lymphocytes
[0199] In this example, 48 cases of tumor infiltrating lymphocytes (TIL) of human hepatocellular carcinoma patients (nine patients lacked paired NIL as a control) and 39 cases of paired paracancerous infiltrating lymphocytes (NIL) were subjected to anti-CD3 and anti-CD3. CD56 fluorescent antibody labeling and flow cytometric analysis (see method 3.1 above).
[0200] For analysis results, see figure 1 As shown, the left picture is a scatter plot of CD3 and CD56 flow staining of TIL and NIL in a representative liver cancer patient and a control benign hemangioma patient; the right picture is TIL in 48 liver cancer patients (9 patients Lack of paired NIL as a control) and CD3 in infiltrating lymphocytes in NIL from 39 patients with liver cancer and 4 controls from patients with benign hepatic hemangioma + CD56 + Summary p...
Embodiment 2
[0202] Example 2, CD3 in TIL and NIL + CD56 + Expression difference of FOXP3 in cells
[0203] In this example, through the staining detection method of intracellular FOXP3 (see the aforementioned method 3.2), the TIL and NIL of hepatocellular carcinoma and the CD3 of the normal control liver group were analyzed. + CD56 + The proportion of cells positive for FOXP3 expression in cell subgroups (because FOXP3 staining belongs to intracellular factor staining, the number of cells required is large. In this example, there are 41 cases of TIL and 37 cases of NIL with FOXP3 staining results).
[0204] The results of the analysis showed that CD3 in TIL compared with NIL + CD56 + The proportion of cells positive for FOXP3 expression in the cell subgroup was significantly increased (22.76±18.61VS 1.476±1.801), NIL was similar to normal liver tissue, and FOXP3 in this cell subgroup + The proportion of cells is very low, which can be counted as a negative proportion ( Figure 2A )....
Embodiment 3
[0206] Embodiment three, CD3 + CD56 + FOXP3 + The enrichment degree of cells in TIL is negatively correlated with the prognosis of patients
[0207] Using the aforementioned "4. Cell sorting" and "5. Statistical analysis" methods, through analysis and summary, the present invention finds that %of FOXP3 in CD3 + CD56 + The level of in TIL was negatively correlated with the patient's disease free survival time and overall survival time, and this correlation was statistically significant ( image 3 ).
[0208] To study %ofFOXP3 in CD3 + CD56 +Whether the factor in TIL is an independent factor related to the prognosis of liver cancer patients was further analyzed in this example using the Cox proportion hazard regression model analysis method, and some raw data of multivariate analysis are shown in the table below. The analysis results prove that this factor can be used as an index representing the overall survival time of patients with liver cancer.
[0209] Multivariate...
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