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image analysis method

An image analysis and digital image technology, applied in the field of image analysis, can solve the problems of not fully utilizing the position information of the positioning mark, the overall calculation amount is large, etc., and achieve the effect of high efficiency and simple overall method.

Inactive Publication Date: 2016-07-20
JINAN LINGKONG INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, this implementation does not make full use of the position information of the positioning marker, and the overall calculation amount is relatively large if other methods are used, and it has no positive impact on accurate positioning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0057] An image analysis method, using the threshold method for the obtained digital image, and positioning the biochip according to the pre-marked highlighted pixel points as the positioning reference point, and then determining each detection point according to the pre-set biochip attribute information Then, the threshold method is used to obtain the sub-positioning reference points of each effective area according to the pre-marked highlighted pixels, and the spot position of the substance to be measured is located and marked according to the sub-positioning reference points of the effective area, and finally the determined The position of the spot, the signal value of the detected spot position is compared with the standard to obtain the analysis result.

[0058] principle:

[0059] The positioning reference point and the sub-location reference point are located in the upper left corner of the corresponding marked area, which meets the general scanning method and can quick...

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Abstract

The invention discloses an image analysis method. The method comprises the steps as follows: for an acquired digital image, a biological chip is positioned by taking a pre-tagged highlight pixel as a positioning datum point with a threshold value method; according to preset attribute information of the biological chip, effective areas of all detection points are determined; further sub positioning datum points of all the effective areas are acquired according to the pre-tagged highlight pixel with the threshold value method; the light spot position of a to-be-detected object is positioned and identified according to the sub positioning datum points of the effective areas; and finally, the determined light spot position is detected, and a signal value for detecting the light spot position and a standard substance are compared, so that an analysis result is obtained. According to the image analysis method, signal values of all detection holes / points in a result image reflected by the biological chip can be accurately acquired.

Description

technical field [0001] The invention relates to an image analysis method, in particular to an information number analysis method specially applied to biochip imaging. Background technique [0002] Biochips, also known as DNA chips or gene chips, are the crystallization of the combination of DNA hybridization probe technology and semiconductor industry technology. This technology refers to immobilizing a large number of probe molecules on the support and hybridizing with fluorescently labeled DNA sample molecules, and obtaining the number and sequence information of sample molecules by detecting the hybridization signal intensity of each probe molecule. [0003] Biochip technology originated from the hybridization of nucleic acid molecules. The so-called biochips generally refer to micro-array hybrid chips (micro-arrays) of biological information molecules (such as gene fragments, DNA fragments or polypeptides, proteins, sugar molecules, tissues, etc.) immobilized at high de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/00
Inventor 孙健李鑫凌郑拓
Owner JINAN LINGKONG INFORMATION SCI & TECH