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A kind of Saccharomyces cerevisiae and its screening culture method and method for soybean meal fermentation

A technology of Saccharomyces cerevisiae and a cultivation method, applied in the field of microorganisms, can solve the problems such as the decline, influence, and destruction of the digestibility of soybean meal by animals, and achieve the effects of stabilizing the quality of fermented soybean meal, reducing animal diseases, and improving the effect of attracting food.

Active Publication Date: 2018-07-31
湖北邦之德牧业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their existence, on the one hand, they can inhibit certain digestive enzymes in animals or complex with nutrients to form indigestible components, which will reduce the digestibility of soybean meal and the absorption rate of animals; on the other hand, they can inhibit certain digestive enzymes in animals Some organs play a destructive role, causing adverse effects on the physiology, growth and health of animals

Method used

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  • A kind of Saccharomyces cerevisiae and its screening culture method and method for soybean meal fermentation
  • A kind of Saccharomyces cerevisiae and its screening culture method and method for soybean meal fermentation
  • A kind of Saccharomyces cerevisiae and its screening culture method and method for soybean meal fermentation

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Embodiment 1

[0037] Example 1: It is a basic example of Saccharomyces cerevisiae of the present invention. A kind of Saccharomyces cerevisiae, its classification is named: Saccharomyces cerevisiae BZD-02 ( Saccharomyces cerevisiae ), the preservation date is: July 9, 2013, the preservation unit is: China Type Culture Collection CCTCC, the preservation center preservation number is: CCTCC NO: M 2013321; the saccharomyces cerevisiae BZD-02, its morphological characteristics are mainly : 1) On YPD medium, the colony is milky white, the surface of the colony is smooth, moist, and viscous, the middle of the colony has a conical protrusion, the texture of the colony is uniform, and the colors of the front and back, the edges and the center are uniform; 2) Microscopic examination of the bacteria The result is oval, budding.

[0038] The optimum growth temperature of the saccharomyces cerevisiae is 25-28°C, but it has good growth and reproduction ability at 10°C, and is suitable for the effective t...

Embodiment 2

[0039] Example 2: It is a basic example of the screening method of Saccharomyces cerevisiae of the present invention described in Example 1. The steps include: firstly formulating soybean meal and molasses into a matrix in a certain ratio, and then burying the matrix in the soil around the winery. After 10-20 days of enrichment, it is separated and purified to obtain a strain of Saccharomyces cerevisiae BZD-02.

Embodiment 3

[0040] Embodiment 3: It is a further embodiment of the screening method described in the above-mentioned embodiment 2, and the matrix is ​​calculated by weight: soybean meal: molasses = 3 to 5:1.

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Abstract

The present invention relates to a kind of Saccharomyces cerevisiae and its screening culture method and method for fermenting soybean meal. Saccharomyces cerevisiae BZD‑02 (Saccharomyces cerevisiae BZD‑02), its morphological characteristics are mainly: 1) On YPD medium, the colony is milky white , the texture of the colony is uniform, and the color of the front and back, the edge and the central part are uniform; 2) The result of the microscopic examination of the bacteria is oval. The screening step includes: mixing soybean meal and molasses in proportion to form a substrate, burying the substrate in soil for enrichment, and then separating and purifying. The cultivation of Saccharomyces cerevisiae includes making medium, culturing strains and low temperature screening. The method for Saccharomyces cerevisiae to ferment soybean meal includes inoculation and fermentation culture. Advantages of the present invention: the strain can grow rapidly in the culture medium, can increase the crude protein content of soybean meal, degrade anti-nutritional factors such as raffinose and stachyose in soybean meal, improve the quality of fermented soybean meal, and increase stability; fermented soybean meal can replace fish meal Such as animal protein feed, improve immunity, prevent and reduce animal diseases, and promote healthy farming.

Description

Technical field [0001] The invention relates to a kind of brewer's yeast, a screening and culturing method thereof and a method for soybean meal fermentation. Specifically, it is a low-temperature saccharomyces cerevisiae strain, a screening and culturing method thereof, and a method for soybean meal fermentation, which are particularly suitable for preparing high-quality fermented soybean meal products, and belong to the field of microbiological technology. Background technique [0002] Soybean meal is a by-product obtained by extracting soybean oil from soybeans. Its crude protein content is high, generally 43% to 45%, with balanced amino acid composition and high nutritional value. Compared with cottonseed meal, peanut meal, rapeseed meal and other animal and vegetable oil meal feed products, soybean meal has the largest output and the most versatile. Soybean meal is an important source of food protein in poultry and swine industry, especially it contains lysine, which is eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18A23K10/30A23K10/18C12R1/865
Inventor 吴晓峰葛向阳李俊
Owner 湖北邦之德牧业科技有限公司
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