Polygalacturonic acid novel application
A polygalacturonic acid and application technology, applied in the field of medicine, can solve problems such as polygalacturonic acid rheumatoid arthritis that have not yet been seen, and achieve the effects of wide application range, good specificity and high sensitivity
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Embodiment 1
[0055] Example 1: Detection of PGA-specific antibody content in serum of healthy people and patients with rheumatoid arthritis
[0056] 1. Experimental materials
[0057] Polygalacturonic acid (PGA, 81325) was purchased from Sigma, USA; Epoxy-activated Sepharose6B and CN-Br-activated Sepharose4B were purchased from Pharmacia Biotech; horseradish peroxidase (HRP)-labeled goat anti- Human IgG and IgA antibodies were purchased from SBA Company.
[0058] Serum samples: Serum samples from 150 healthy people were provided by Beijing Red Cross Blood Center; 80 cases of active RA patients, 20 cases of early RA patients, 49 cases of osteoarthritis, 32 cases of ankylosing myelitis, 13 cases of psoriatic joints Serum samples from patients with inflammation were provided by Peking University Second Hospital.
[0059] Equipment and consumables: micro-sampler from Eppendorf; constant-flow pump from Beijing Liuyi Instrument Factory; 4°C refrigerator; -80°C low-temperature refrigerator; mic...
Embodiment 2
[0067] Example 2: The ROC curve of the PGA-specific antibody content comparison in the serum of healthy people and patients with rheumatoid arthritis
[0068] According to the levels of PGA-IgA and PGA-IgG in healthy people, active RA patient group and early RA patient group in Example 1, ROC curves were drawn respectively. Taking the content of PGA-specific antibody in the serum of healthy people as a contrast in Example 1, the content of PGA-specific antibody in the serum is analyzed to the RA active stage patient group and the early stage RA patient group, determine the upper and lower limits of the measured value, group interval As well as the cutoff point, the cumulative frequency distribution table is listed according to the selected group distance interval, and the sensitivity, specificity and false positive rate (1-specificity) of all cutoff points are calculated respectively. Taking sensitivity as the ordinate to represent the true positive rate, (1-specificity) as th...
Embodiment 3
[0070] Example 3: Comparison of PGA Antibody with RF and CCP-Abs Specificity and Sensitivity
[0071] Quanta Lite CCP3IgG ELISA kit (purchased from INOVA Diagnostics, USA) was used to measure the CCP-Abs content of healthy people, RA active patient group and early RA patient group: the content of CCP-Abs was determined by IMMAGE (Immunochemistry system machine kit) (purchased from U.S. Beckman company) detect the content of RFs in healthy people, RA active stage patient group and early RA patient group. ROC curve is drawn respectively according to the method for embodiment 2, calculates the area under the ROC curve AUC. And calculate different according to the following formula Specificity and sensitivity of detection indicators. Sensitivity = number of true positives / (number of true positives + number of false negatives) * 100%, which represents the rate of correctly judging patients. Specificity = number of true negatives / (number of true negatives + false positives numbe...
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