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Method for improving cordycepin output in cordyceps militaris fermentation broth

A technology of Cordyceps militaris and fermentation liquid, which is applied in the field of fermentation technology for improving the yield of cordycepin, can solve problems such as low yield of cordycepin, and achieve the effects of simple fermentation process, less addition, and no toxic and side effects.

Active Publication Date: 2013-12-04
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage is that the yield of cordycepin in the fermentation broth is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The strain of Cordyceps militaris preserved by the China General Microorganism Culture Collection and Management Center was used, and the preservation number was CCGMC3.4655.

[0025] Seed medium composition: sucrose 22g / L, yeast extract 8g / L, KH 2 PO 4 1.3g / L, MgSO 4 0.60g / L; liquid fermentation medium composition: sucrose 15g / L, yeast extract 5g / L, peptone 5g / L, KH 2 PO 4 1.3g / L, MgSO 4 0.60g / L. The mycelium block was inoculated in a 2L shake flask containing 1.2L seed medium, and fermented in a rotary shake flask cabinet with a fermentation temperature of 22°C and a rotation speed of 150r / min for 8 days to obtain a seed liquid, which was adjusted with sterile water. to a concentration of 2.1g / L (based on the dry weight of the bacteria), and then inoculate 120mL of seed liquid into a 2L shake flask containing 1.2L of fermentation medium. Fermentation temperature is 25°C, rotation speed is 145r / min, ferment for 2 days first, then add 2.0g / L oleic acid and adenine...

Embodiment 2

[0028] The strain of Cordyceps militaris preserved by the China General Microorganism Culture Collection and Management Center was used, and the preservation number was CCGMC3.4655.

[0029] Seed medium composition: sucrose 24g / L, yeast extract 6g / L, KH 2 PO 4 1.3g / L, MgSO 4 0.60g / L; composition of liquid fermentation medium: sucrose 16g / L, yeast extract 6g / L, peptone 6g / L, KH 2 PO 4 1.3g / L, MgSO 40.60g / L. Inoculate the mycelium block into a 2L shake flask containing 1.2L seed medium, and ferment for 7 days in a rotary shake flask cabinet with a fermentation temperature of 23°C and a rotation speed of 160r / min to obtain a seed liquid, which is adjusted with sterile water. to a concentration of 2.3g / L (based on the dry weight of the bacteria), and then inoculate 120mL of seed liquid into a 2L shake flask containing 1.2L of fermentation medium. Fermentation temperature is 24°C, rotation speed is 160r / min, ferment for 2 days first, then add 2.0g / L oleic acid and adenine mix...

Embodiment 3

[0032] The strain of Cordyceps militaris preserved by the China General Microorganism Culture Collection and Management Center was used, and the preservation number was CCGMC3.4655.

[0033] Seed medium composition: sucrose 22g / L, yeast extract 10g / L, KH 2 PO 4 1.3g / L, MgSO 4 0.60g / L; liquid fermentation medium composition: sucrose 24g / L, yeast extract 5g / L, peptone 5g / L, KH 2 PO 4 1.3g / L, MgSO 4 0.60g / L. Inoculate the mycelium block into a 2L shake flask containing 1.2L seed medium, and ferment for 7 days in a rotary shake flask cabinet with a fermentation temperature of 25°C and a rotation speed of 170r / min to obtain a seed liquid, which is adjusted with sterile water. to a concentration of 2.0g / L (based on the dry weight of the bacteria), and then inoculate 120mL of seed liquid into a 2L shake flask containing 1.2L of fermentation medium. Fermentation temperature is 25°C, rotation speed is 180r / min, ferment for 2 days first, then add 2.0g / L oleic acid and adenine mixt...

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Abstract

The invention discloses a method for improving cordycepin output in cordyceps militaris fermentation broth. A cordyceps militaris strain (cordyceps militaris) preserved by the China general microbiological culture collection management committee is adopted, and the strain preservation number is CCGMC3.4655. The method includes the steps of inoculating a seed solution of the cordyceps militaris, performing submerged fermentation for 2 to 5 days in a rotary shake-flask cabinet at the temperature of 20 DEG C to 26 DEG C and at the rotating speed of 140 rounds per minute to 190 rounds per minute, refilling 1.0g / L to 3.0g / L of the mixture of oleic acid and adenine, wherein the mass ratio of the mixture to the fermentation broth is 1:3 to 3:1, and continuously fermenting for 4 to 7 days under the conditions that the temperature ranges from 20 DEG C to 26 DEG C and the rotating speed ranges from 140 rounds per minute to 190 rounds per minute. According to the method, the cordycepin output reaches up to 813.7mg / L.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a fermentation process for increasing the yield of cordycepin. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a model species of Ascomycota, Ergotaceae, Ergotaceae, and Cordyceps. Hemostasis and phlegm, nourishing and strong effects. Compared with Cordyceps militaris, Cordyceps militaris has the following advantages: First, Cordyceps militaris is a model species of the genus Cordyceps, widely distributed, recognized and accepted by scholars all over the world; The third is that Cordyceps militaris contains cordycepin (Cordycepin, 3′-deoxyadenosine), and its unique pharmacological effects have increasingly attracted great attention from the pharmaceutical community. Because Cordyceps militaris has the above advantages, it has become the leader among the Chinese medicinal Cordyceps fungi o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C07H19/16C05G3/00
Inventor 刘高强朱朝阳陈慧王晓玲毛绍名彭宽周国英
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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