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Embryo water extract extracted from animal embryo skin and muscle, and extraction method and applications thereof

A water extract and embryonic technology, applied in the field of embryonic extraction, can solve the problems of difficulty in industrial production and long-term storage, low embryonic content, low activity of sheep embryonic, etc., and achieves the effect of facilitating industrial scale production and absorption. Good, improve the effect of embryonic content

Active Publication Date: 2015-05-27
扬州谢馥春古典化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth noting that the extraction of sheep placenta is a very complicated biotechnological process, which can only be done in the laboratory today, and it is difficult to carry out large-scale industrial production and long-term storage
In our country, some laboratories use the method of proteolytic enzymatic hydrolysis to extract sheep placenta. Although this method can greatly increase the total protein content in the hydrolyzate, it is found that the main increase in the detected protein is that there is no biological regulation. Active amino acids and active peptide fragments, but the content of embryonic elements that can really play a regulatory role is not high, indicating that the activity of sheep embryonic elements extracted after proteolytic hydrolysis treatment is low, and proteolytic enzymes have a negative effect on sheep embryos. The element has a certain destructive effect, and now this extraction method is gradually eliminated by the new extraction method

Method used

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Embodiment Construction

[0033] 1. Extraction method of water-soluble embryonic element with molecular weight less than 5000:

[0034] 1. Place fresh animal embryos taken out at the age of 30 to 145 days at -15 to -25°C for cryopreservation.

[0035] 2. Wash the cryopreserved animal embryos with double distilled water, and separate the skin and muscle of the embryos. The separated embryonic skin and muscle are then crushed mechanically for use.

[0036]3. Soak the mechanically pulverized embryonic skin and muscle in double distilled water at a temperature of 0-5°C for 20-30 minutes under normal pressure. The mixing weight ratio of double distilled water to embryo viscera is 1-4:1.

[0037] 4. Repeatedly freeze and thaw the mixture formed by mixing double distilled water with embryonic skin and muscle for 2 to 4 times. The first thawing temperature is ≤10°C.

[0038] 5. Treat the mixed solution after the above-mentioned repeated cycles of freezing and thawing with a refrigerated centrifuge at 4000-...

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Abstract

The invention discloses an embryo water extract extracted from animal embryo skin and muscle, and an extraction method and applications thereof, and belongs to the field of extraction technology of embryo extract from animal embryos, especially from embryo skin and muscle of animals such as sheep, pig and dog. The extraction method comprises following steps: embryo skin and muscle obtained from frozen preserved animal embryos are immersed in double distilled water, then are subjected to mechanical pulverization and repeated freezing and thawing, and are processed by a refrigerated centrifuge for 10 to 15min so as to obtain a water extracting solution; the pH value of the water extracting solution is adjusted to 2 to 4, and then is subjected to refrigerated centrifugation to remove a sediment and obtain a supernatant; the pH value of the supernatant is adjusted to 4 to 6, and then is subjected to refrigerated centrifugation to remove a sediment and obtain a water solution; the water solution is subjected to ultrafiltration so as to obtain the water soluble embryo extract with a molecular weight below 5000D. Embryo resources of the embryo water extract are wide; embryos are easily available; and the embryo water extract is suitable for industrialized large-scaled production, and is capable of ensuring substances in the extract to be easily absorbed by human skin and mucosal tissues.

Description

technical field [0001] The invention relates to the technical field of extracting placebosin from animal embryos, especially from skin and muscle of animal embryos such as sheep, pigs and dogs. Background technique [0002] In 1912, Swiss scientist Professor Karl won the Nobel Prize in Medicine for discovering for the first time that there is a magical substance in sheep embryo cells that can revitalize human cells, that is, sheep placenta, and found the pure natural animal product sheep placenta that can delay human aging. So that people's dream of pursuing youth and prolonging life becomes a reality. [0003] According to scientific research, the number of biological cell divisions is related to lifespan. For example, a chicken with a lifespan of 30 years has 25 cell divisions, while a mouse with a lifespan of only 3 years has only 12 divisions. The root cause of human aging is the aging of the most basic unit cells that make up the living body; as the age increases, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/98A61K8/64A23L1/29A61K35/48A61P37/04A61Q19/00A23L33/00A61K35/54
Inventor 周斌叶满红
Owner 扬州谢馥春古典化妆品有限公司
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