Novel composite protein source and its application in turbot feed

A compound protein and compound protease technology, applied in the field of new compound protein source and its application in turbot feed, can solve the problems of turbot's reduced feeding rate, decreased autoimmunity, intestinal flora imbalance, etc. Achieve the effect of improving intestinal flora, reducing application and reducing feed cost

Active Publication Date: 2014-01-01
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a new type of composite protein source and its application in turbot feed, which can solve the problem of reduced feeding rate, slow growth, intestinal flora imbalance and The problem of decreased autoimmunity, making up for the shortcomings of existing technologies

Method used

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Examples

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Effect test

preparation example Construction

[0014] The preparation of the above-mentioned small-molecule fishmeal active substance is made of Pacific pollock fish fillet, cleaned, minced by a meat grinder, added Bacillus complex protease, and then mixed with water and Bacillus complex protease, the amount of Bacillus complex protease E / S=90AU , Enzymolysis temperature: 50℃~60℃, initial pH=7.5, material-water ratio: 1:1, enzymolysis for 30min. Then the enzymolysis solution is heated to above 100°C to inactivate the Bacillus complex protease, and the spurs are separated by filtration. The enzymolysis solution is further centrifuged in three phases to obtain soluble water phase, insoluble precipitate and fish oil respectively, and the soluble water phase is purified by ultrafiltration membrane ultrafiltration. After spray-drying, the small-molecule fish meal active substance was obtained. The protein content was 91.3%, fat 0.38%, ash content 7.8%, taurine 14%, anserine 20.4%, molecular weight less than 100Da accounted for 1...

Embodiment

[0019] In order to verify the application effect of the novel composite protein source of the present invention, the above-mentioned feed A of the present invention and commercial feed B (shengsuo feed, 45% protein, 12% fat) were used to conduct a comparative test, and both feeds were extruded feeds. The experiment was carried out in Tianyuan Aquatic Products Co., Ltd., Yantai Development Zone. Six cement ponds were selected, and three parallel feeds were set up for each type of feed. Healthy turbot with the same specifications were selected and put into the cement ponds. The cycle is 4 months. The initial mass was (4.80±0.11) g. The number of vibrio and lactic acid bacteria in the intestinal tract of turbot were measured after breeding (see Table 1 for the measurement results). There was no significant effect on the number of bacteria, but it significantly reduced the number of vibrio in the intestine (P<0.05), significantly reduced the number of lactic acid bacteria in the i...

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PUM

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Abstract

The invention relates to a novel composite protein source and its application in a turbot feed, and belongs to the aquatic animal nutrition and feed fields. The novel composite protein source comprises 5-6wt% of micro-molecular fishmeal active substances, 10-15wt% of fishmeal, 10-15wt% of soybean protein concentrate, 20-25wt% of soybean meal, 20-25wt% of corn protein powder, 5-18wt% of wheat gluten powder and 10-15wt% of peanut meal. Compared with commercial feeds, the novel composite protein source has the advantages of reduction of the total number of intestinal vibrio in the turbot, promotion of the improvement of the body immunity, substantial improvement of the growth performance, and reduction of the consumption of the fishmeal in the turbot feed.

Description

technical field [0001] The invention belongs to the field of aquatic animal nutrition and feed, and in particular relates to a novel composite protein source and its application in turbot feed. Background technique [0002] The most urgent problem brought about by the rapid growth of population is to provide food for human beings. As a kind of high-value food with high protein and low fat in food, people's demand for it is naturally increasing day by day. Protein is the most important nutrient in aquatic animal feed, and it is also the key to the cost of feed. Aquatic animals, especially marine aquatic animals, have high requirements for protein content in feed and are highly dependent on animal protein sources, resulting in generally high feed costs for aquaculture animals (Gatlin et al., 2007). Fishmeal is the main protein source of carnivorous fish feed, and also the main component of feed cost. Due to the increase in demand for fishmeal and relatively stable supply, fis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/18A23K1/14A23K1/00
Inventor 梁萌青郑珂珂卫育良
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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