A Phomopsis fungus and its application in the preparation of fungal wood
A technology of Phomopsis and fungi, applied in the field of microorganisms, can solve the problems of decay or discoloration, increased cost, and long time for the formation of natural fungus wood, and achieves the effect of good infection effect and elimination of dead corners.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0066] The preparation method may also include a post-processing step, which is to scrape off the mycelia on the surface of the fungal wood obtained after inoculation culture, wash and dry to a moisture content of 8-10%.
[0067] The wood-based material in step B is any one or more of logs, solid wood blocks, planks, veneers, wood flakes, sticks, and special-shaped wood products.
[0068] The tree species of the wood-based material in step B can be any tree species.
[0069] The tree species of the wood-based material described in step B are preferably poplar tomentosa, birch birch, alder wood, and pine simao.
[0070] The wood-based material described in step B can be processed into any shape as required before inoculation.
[0071] The sterilization described in step B is to sterilize the wood-based material in a high-pressure steam sterilizer at 121° C. for more than 20 minutes.
[0072] The sterilization described in step B is to place the wood-based material in a cultur...
Embodiment 1
[0080] —— Phomopsis fungi ( Phomopsis sp.) Acquisition, identification and preservation of J9GAN.
[0081] (1) Phomopsis fungus ( Phomopsis sp.) Acquisition and identification of J9GAN.
[0082] The Phomopsis fungus described in the present invention is isolated from dead branches of living trees. The samples were collected from Jindian Park, Kunming City, Yunnan Province. The dead branches of the living standing trees of broad-leaved wood with lines and patterns were collected. The length was about 3 cm and the diameter was about 1.5 cm. They were stored at 4°C for later use.
[0083] Cut the collected samples of decayed dead branches into small pieces of 0.3~0.5cm×0.3~0.5cm×0.2~0.4cm with a scalpel, soak them in 75% alcohol for 10~15s, and then soak them in 0.1% mercuric chloride for disinfection 4~6min, and then washed 3 times with sterilized distilled water. Place it in PDA medium and culture it in the dark at 28°C for 10 days until mycelium grows; then the colony is ...
Embodiment 2
[0094] —— Phomopsis fungi ( Phomopsis sp.) J9GAN to prepare fungal wood.
[0095] (1) Preparation of fungal grain wood.
[0096] First, Phomopsis fungus (Phomopsis sp.) J9GAN was inoculated on PDA liquid medium and cultured on a dark shaker at 26°C for 9 days to form mycelium with a diameter of 1-3 cm and a suspension of mycelium spores. The composition of PDA liquid medium is calculated in g / L: potato 200, glucose 18, pH 6.5-7.0.
[0097] Then, the poplar tomentosa log to be inoculated was cut into small pieces of 2cm x 2cm x 3cm, and placed in a culture bottle with a bottom diameter of 6cm and a height of 11cm. Next, pour the vermiculite into the culture bottle until it covers the wood block, add a small amount of water to moisten the wood block and vermiculite, but no excess water overflows, cover the bottle cap, put it in a high-pressure steam sterilizer, and sterilize it at 121°C. Bacteria for 60 minutes.
[0098] Clamp the wooden block and put it into the fungal sus...
PUM
Property | Measurement | Unit |
---|---|---|
area | aaaaa | aaaaa |
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com