Closed chromatographic test paper detection device as well as using method thereof

Abstract

The invention discloses a closed type chromatography test paper detection device and an application method of the closed type chromatography test paper detection device. The closed type chromatography test paper detection device comprises a solution tube, a connection tube and a test paper tube, wherein the tube body bottom of the solution tube is provided with a bottom structure with shear marks; the inner wall circumference surface of the tube body of the solution tube is provided with two grooves, one groove allows repeated opening of a cover of the solution tube, and the other groove reversely hooks the cover to form the closed environment; the inner circular wall of a counter bore-shaped connector of the connection tube matched with the solution tube is provided with a convex sealing ring, the convex sealing ring is matched with the groove of the solution tube and then hooks reversely to form the closed environment; the bottom of the connector of the connection tube, for insertion of the solution tube, is provided with a structure interacting with the bottom structure of the solution tube to release solution; the connector between the connection tube and the test paper tube is internally provided with the convex sealing ring, the convex sealing ring is matched with the groove outside the test paper tube and then reversely hooks to form the closed environment; and moreover, other solution tubes can be stacked and sleeved on the solution tube so as to form multiple sleeves. The closed type chromatography test paper detection device is small in volume, can realize complete closure, and can completely prevent influence and pollution to the environment caused by the sample in the chromatography test paper sample solution.

Description

technical field [0001] The invention relates to a chromatography test paper detection device, in particular to a closed chromatography test paper detection device; in addition, the invention also relates to a method for using the device. Background technique [0002] The lateral flow chromatography test paper technology (Lateral Flow, US6,485,982) invented by Charlton in 1988 triggered a revolution in immunological diagnosis. The solid-phase immunoreaction on the surface has achieved unprecedented simplicity, rapidity, precision and cheapness. Subsequently, colloidal gold immunochromatographic test paper was promoted to widely used rapid medical in vitro diagnostic reagents, and then also to the field of rapid detection of food safety and biosafety. At the same time, other dry biochemical test papers also began to use chromatography test paper. Among the detection items of these chromatography test papers, some detection substances have harmful effects on operators and the...

AI Technical Summary

Problems solved by technology

The first is to create a closed environment in the process of detecting nucleic acid amplification products, such as: electrophoresis is carried out in a closed negative pressure room, and the reaction tube is not opened during the whole process of real-time fluorescent PCR, but these methods require strict experimental conditions And expensive experimental equipment, not suitable for popularization to grassroots users

The second is to incorporate dUTP in the nucleic acid amplification reaction, and to treat the test equipment and environment with UNG enzyme after the reaction is completed and before the next nucleic acid amplification, but this method is cumbersome to operate and has a certain probability of contamination, which limits this method. application of strategies

However, the disadvantages of this device are: 1. The device is large in size and is not suitable for large-scale production and transportation of finished products; 2. The test strips in the device are complicated to install and are not suitable for large-scale test paper assembly and production; 3. After the device is restarted and used , can be reopened easily and without traces, so the nucleic acid amplification product is only relatively closed. If the device that has been reacted is opened unintentionally, the contamination of the amplification product is inevitable; 4. The device In this method, only a single reaction solution can be detected, but multiple reaction solutions cannot be detected, that is, the detection of multiple samples cannot be completed on one test strip in the form of multiple reaction solutions

This is not conducive to environmental protection, but also increases the difficulty and cost of injection molding production

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