System, especially in the medical field, for quantitative chemical analysis of samples with calibration of instrument response and corresponding method
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[0035] The main disadvantage of the second method and approach is that it requires the use of extremely expensive isotope standards, which limits the practical use of the method to centers that regularly process large numbers of samples, such as hospitals, forensic analysis centers, quality control and similar experiments room
[0041] It is worth noting that the calibration of the instrument used for the analysis must be repeated each time; in other words, it must be performed before analyzing each sample, which, as already stated, constitutes an important drawback of the prior art
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example 1
[0196] Example 1 - Analysis of cocaine and its metabolites
[0197] In this example, the PROSAD technique was used to quantify cocaine and its metabolite benzoylecgonite in urine samples.
[0198] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulisation gas flow rate was 2 L / min.
example 2
[0199] Example 2 - Testosterone analysis in plasma samples
[0200] The PROSAD system was used to analyze testosterone in human plasma.
[0201] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulizing gas flow rate was 2 L / min.
example 3
[0202] Example 3 - Analysis of Tacrolimus in Whole Blood Samples
[0203] In this example, the PROSAD system was used to assay an immunosuppressant called tacrolimus (anti-rejection drug) contained in human plasma.
[0204] A binary chromatographic gradient was used comprising phase A) H2O + 0.05% formic acid and phase B) CH3CN + 0.05 formic acid. Given the time T=0 at the time of injection, %B was 15%. Maintain this condition for 2 minutes. After this interval, %B increased to a value of 70% within 8 minutes. Initial conditions were restored over the next two minutes. The instrument acquisition time was set at 24 minutes. A ThermolEctron C8 150×1 mm chromatographic column was used. The surface potential, electrospray potential and surface temperature were 50V, 0V and 110°C, respectively. The nebulizing gas flow rate was 2 L / min.
[0205] Finally, for the sake of completeness, for each of the above application examples, the % measurement accuracy error and the instrum...
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Abstract
Analytical systems (10, PROSAD) especially but not only in the medical field for quantitative chemical analysis of samples (A, A-1, A-2, . A1) An instrument or device (30) for detecting the amount of the target analyte ([x], [y], [z]) present in, which in turn includes a chromatographic system (31), an ion source (32) and a mass spectrometer ( 33); a data processing system (40, 42, 43, 44), designed to process the quantitative data of the target analyte in the analyzed sample detected by the detection device (30); and an innovative database (41), which contains the Correction and control data and coefficients (K, C, K1) for calibrating and correcting the instrument response of a specific detection device (30) for analyzing samples, wherein said correction and control data and coefficients are the database before actual sample analysis determined and obtained in the initial phase (51, 52), wherein the samples to be analyzed are prepared by universal dilution solutions to minimize the corresponding matrix effects, and wherein by processing the detection device while taking into account the correction and control data and coefficients contained in the database (ie mass spectrometer) detected quantitative data (Q), the data processing system determines the quantitative data of the target analyte present in the analyzed sample. The system for the quantitative analysis of samples according to the present invention exhibits a significant advantage compared to currently used analysis systems, that is, it does not require continuous and expensive Properly use commercial standards for the target analytes to be quantified.
Description
technical field [0001] The present invention relates generally to the field of quantitative chemical analysis systems and devices for samples, typically but not exclusively in the medical field, and more particularly to such analytical systems for quantitative chemical analysis of samples, characterized by a novel and advantageous calibration system, It is designed to calibrate the instrument response of a particular instrument or device in an analytical system that detects the amount of target analyte present in the various samples being analyzed. [0002] The invention also relates to a corresponding method for the quantitative analysis of samples, especially in the medical field, with calibration of the instrumental response of a particular instrument or device used to detect quantitative data of target analytes in the various analyzed samples. Background technique [0003] Modern analytical chemistry, in its practical application, needs to continuously enhance the quanti...
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