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Method for extracting and detecting thiourea dioxide in food

A technology of thiourea dioxide and extraction method, which is applied in the field of food analysis, can solve the problem that the sensitivity cannot reach trace detection, etc., and achieve good economic and social benefits, less matrix interference, and a simple method

Active Publication Date: 2014-02-19
GUANGZHOU QUALITY SUPERVISION & TESTING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of thiourea dioxide has been decomposed after being used as a bleaching agent, and its residual amount in food is trace, and it is easily interfered by other substrates. The sensitivity of the above method cannot meet the requirements of trace detection.

Method used

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  • Method for extracting and detecting thiourea dioxide in food
  • Method for extracting and detecting thiourea dioxide in food
  • Method for extracting and detecting thiourea dioxide in food

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0056] This embodiment is an example of extracting and measuring the content of thiourea dioxide in the stuffing material, comprising the following steps:

[0057] (1) Preparation of thiourea dioxide matrix calibration working solution:

[0058] Weigh 0.010g of thiourea dioxide (accurate to 0.0001g), dissolve it in ultrapure water, transfer it to a 100mL volumetric flask, add acetonitrile to constant volume, and prepare a 100mg / L standard stock solution of thiourea dioxide;

[0059] Select the negative stuffing sample, and dilute the thiourea dioxide standard stock solution step by step into a series of matrix calibration working solutions with concentrations of 1.0, 2.0, 5.0, 20.0, 50.0, and 200.0 μg / L according to the test solution treated in step (2).

[0060] (2) Preparation of extract:

[0061]Weigh 5.0g (accurate to 0.01g) sample (stuffing material) into a 50mL plastic centrifuge tube, add 10mL acetonitrile, vortex for 1min, ultrasonically extract for 10min, centrifuge ...

Embodiment 2

[0093] This embodiment is the extraction and determination example of the content of thiourea dioxide in the control / standardized sample of Wuren stuffing, and the results are as follows image 3 , Figure 4 shown.

[0094] 1. Control sample:

[0095] Take the Wuren stuffing sample without adding thiourea dioxide, and extract and measure the content of thiourea dioxide in the Wuren stuffing sample according to the extraction and determination method in Example 1. The test results are shown in image 3 .

[0096] Such as image 3 Shown: the control Wuren stuffing sample does not contain thiourea dioxide.

[0097] 2. Spiked sample:

[0098] Take Wuren stuffing samples without adding thiourea dioxide, add thiourea dioxide standard solutions with concentrations of 2.0, 5.0 and 20.0 μg / L respectively, and then extract and measure according to the extraction and determination methods in Example 1. The test results (20.0 μg / L) see Figure 4 .

[0099] The linear equation of t...

Embodiment 3

[0101] This embodiment is an example of extracting and measuring the content of thiourea dioxide in the control / standardized sample of red lotus paste filling, and the results are as follows Figure 5 , Figure 6 shown.

[0102] 1. Control sample:

[0103] Get the red lotus paste filling sample without adding thiourea dioxide, and extract and measure the content of thiourea dioxide in the red lotus paste filling sample according to the extraction and determination method in Example 1, the test results are shown in Figure 5 .

[0104] Such as Figure 5 Shown: The control red lotus paste filling sample does not contain thiourea dioxide.

[0105] 2. Spiked sample:

[0106] Take the red lotus paste filling sample without adding thiourea dioxide, add the standard solution of thiourea dioxide with a concentration of 2.0, 5.0 and 20.0 μg / L respectively, and then extract and measure according to the extraction and determination method in Example 1. The test results ( 20.0μg / L) ...

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Abstract

The invention discloses a method for extracting and detecting thiourea dioxide in food. According to the method, thiourea dioxide in a greasy food substrate or non-greasy food substrate is extracted through such steps as organic solvent dissolution, ultrasonic digestion, centrifugation, back extraction, filtration and the like, and ultra-high performance liquid chromatography-mass spectrum / mass spectrum is carried out in a multi-reaction monitoring (MRM) manner. The sample pre-treatment method disclosed by the invention is simple and is high in recovery rate, small in substrate interference, low in detection limit, high in accuracy and capable of filling an existing blank of thiourea dioxide detecting method in food and providing important technological support for food quality control and food safety detection, so that the method has good economic and social benefits.

Description

technical field [0001] The invention relates to the field of food analysis, in particular to a method for extracting and detecting thiourea dioxide in food. Background technique [0002] Thiourea dioxide, also known as thiourea peroxide, also known as formamidine sulfinic acid (TDO), as an excellent reducing agent, is widely used in textile, printing and dyeing and other chemical processes, such as: as a decolorizing agent for various coloring Decolorization of fibers; used as a bleaching agent for the bleaching of fibers such as paper, wool, and silk, and phenolic resins and tanning materials. The relevant information in the "List of Non-edible Substances and Food Additives That May Be Illegally Added in Food" shows that thiourea dioxide may be illegally used as a filling raw material bleaching agent for the bleaching of baked food filling raw materials, edible Thiourea dioxide can cause headache, nausea, oral and gastric irritation, but there is no relevant standard on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 陈立伟罗海英郭新东吴玉銮侯向昶冼燕萍朱丽萍韩婉清罗东辉王斌
Owner GUANGZHOU QUALITY SUPERVISION & TESTING INST
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