A method for culturing porcine testicular cells without serum and using it to produce swine fever cell vaccine

Abstract

The invention discloses a method for cultivating porcine testicular cells without serum and using the same to produce a swine fever cell vaccine; the method for cultivating porcine testicular cells without serum is as follows: culturing the porcine testicular cells with a medium containing 10% calf serum, When the cells grow to 60%, replace the medium containing 5% calf serum for adaptation and replace the fresh medium when cells die and suspend, and subculture with the medium containing 10% calf serum when the cells are congested 3-5 generations; repeat the above steps, gradually reduce the serum content from 10% to 5%, 2%, 1% until serum-free culture; finally use the extinction dilution method to screen pure cell lines. In the present invention, pig testicular cells are obtained from serum-free acclimatization culture, and the cells can better proliferate classical swine fever virus and be applied to the production of classical swine fever cell vaccine, which not only avoids the risk brought by the use of animal serum, but also makes the composition of the medium clear, Improves lot-to-lot identity and simplifies downstream purification processing.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and relates to a serum-free method for cultivating porcine testicular cells and using the same to produce a swine fever cell vaccine. Background technique [0002] Classical swine fever (CSF) is a highly contagious febrile disease caused by classical swine fever virus (CSFV). In 1984, the World Organization for Animal Health (OIE) listed it as a Class A infectious disease, and it is also designated as a Class I infectious disease in my country. In addition to causing sepsis, swine fever can also cause a series of other clinical manifestations, such as abortion in pregnant sows, fetal deformation, chronic nutritional consumption, lymphocyte and thrombocytopenia, etc. At present, most countries in the world have adopted strict sanitation and epidemic prevention measures and eliminated swine fever-infected pigs to purify pig herds to control and eliminate swine fever. Some developed cou...

AI Technical Summary

Problems solved by technology

However, the use of animal serum in the vaccine production process will lead to increased costs, the risk of introducing foreign proteins, and introducing other viruses and pathogens, which is a thorny problem faced in the production process of cell vaccines