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Avian pentavalent vaccine and preparation method thereof

A vaccine and poultry technology, which is applied in the field of poultry penta vaccine and its preparation, can solve the problems that the product has not been widely used, and there is no research report and use of livestock and poultry penta vaccine.

Inactive Publication Date: 2014-03-05
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the commercial products currently on the market at home and abroad are traditional single vaccines, and only a few double and triple vaccines. Although there are a large number of research reports on quadruple vaccines, their commercial products have not yet been widely used. So far, there is no research report and use of the pentavalent vaccine for livestock and poultry

Method used

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  • Avian pentavalent vaccine and preparation method thereof
  • Avian pentavalent vaccine and preparation method thereof
  • Avian pentavalent vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] Embodiment 1: Preparation of poultry pentavalent vaccine antigen and semi-finished product inspection

[0111] (1) Antigen preparation

[0112] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0113] (1) Inoculation: Take the virus seeds for production, and use sterilized physiological saline to make 10 -4 Inoculate 400 10-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinhole after inoculation, and continue to incubate at 37°C without turning the embryos during the period.

[0114] (2) Culture: Incubation and observation After inoculation of chicken embryos, the embryos were photographed once a day, and 18 chicken embryos that died 60 hours ago were discarded. Thereafter, the embryos were photographed once every 4 to 6 hours, and the dead embryos were taken out at any time until 96 hours, all were taken out, the air chamber was upward, and placed at 4°C for 12 hours to cool.

[0115] (3) Harvesting: Take out the cooled chicken embryos and ...

Embodiment 2

[0166] Embodiment 2: the preparation of vaccine

[0167] 1. Oil phase preparation Take 3840ml of high-quality white oil for injection and 80ml of aluminum stearate, mix them evenly in the oil phase preparation tank and heat to 80°C, then add Siben-8080ml, and keep it for 30 minutes when the temperature reaches 125°C , and set aside after cooling.

[0168] 2 Preparation of water phase: Mix 400ml each of qualified chicken Newcastle disease virus liquid, infectious bronchitis virus liquid, infectious bursal disease virus liquid, viral arthritis virus liquid, chicken egg drop syndrome virus liquid (after mixing Make the Newcastle disease virus content in 0.1ml aqueous phase not less than 10 8.5 EID 50 , the infectious bronchitis virus content is not less than 10 7.5 EID 50 ;Infectious bursal virus content is not less than 10 7.0 TCID 50 ; viral arthritis virus content is not less than 10 7.5 EID 50 , chicken egg drop syndrome virus content is not less than 15log2). Take 0...

Embodiment 3

[0171] Embodiment 3: finished product inspection

[0172] According to the above preparation method, the laboratory trial-produced a batch of pentavalent vaccine vaccine for poultry about 6000ml, the batch number is 2011001.

[0173] 1. traits

[0174] Appearance: Milky white emulsion.

[0175] Dosage form: water-in-oil type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it will not spread.

[0176] Stability: Take 10ml of the vaccine and put it into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, the water phase precipitated at the bottom of the tube is 0.1ml, which meets the regulations.

[0177] Viscosity: Use a 1ml straw (the inner diameter of the lower mouth is 1.2mm, and the inner diameter of the upper mouth is 2.7mm) to absorb 1ml of vaccine at about 25°C, let it flow out vertically and naturally, and record the time required for the outflow of 0.4ml to be within 8 seconds.

[0178] 2. The filli...

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Abstract

The present invention discloses a Newcastle disease, infectious bronchitis, infectious bursal disease, viral arthritis and egg drop syndrome inactivated pentavalent vaccine, and a production method thereof. The antigens of the pentavalent vaccine comprise a Newcastle disease virus La Sota strain, an infectious bronchitis virus K136 strain, an infectious bursal disease virus BJQ902 strain, a viral arthritis T98 strain and an egg drop syndrome virus Jing 911 strain. According to the present invention, the multi-stage continuous concentration process and the homogenizer emulsification process are adopted during the preparation process, the inactivated vaccine for concurrently preventing and treating the five chicken epidemic diseases is firstly prepared, characteristics of rapid antibody generation, high titer and long protection period are provided after immunizing animals, and epidemic disease occurrence and spread are reduced.

Description

technical field [0001] The invention belongs to the technical field of new biomedicine for livestock and poultry, and in particular relates to a pentavalent vaccine for poultry and a preparation method thereof. Background technique [0002] Newcastle disease (ND) is a highly contagious disease caused by a paramyxovirus, also known as Asian chicken plague or pseudo chicken plague. Sepsis often occurs during the course of the disease, and the main clinical features are dyspnea, diarrhea, neurological disorders, and mucosal and serosal hemorrhage. Chickens of all breeds and ages can be infected, young chickens and middle-aged chickens are more susceptible to infection, and the mortality rate is extremely high, often causing a devastating blow to the poultry farming industry. [0003] Chicken infectious bronchitis (Infectious Bronchitis, IB) is an acute and highly contagious respiratory infectious disease of chickens caused by a virus belonging to the genus Coronaviridae. Its c...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61P31/14A61P31/20A61K39/17A61K39/215A61K39/12A61K39/15
Inventor 夏广欣陈冰康亚男李建丽吴全忠何召庆毛雅元杨保收梁武李守军
Owner TIANJIN RINGPU BIO TECH
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