30 results about "Egg drop syndrome virus" patented technology

The invention discloses a GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases. The invention provides a GeXP detection primer group for identifying or assisting to identify duck infectious disease pathogens, wherein the primer group consists of a primer pair A, a primer pair B, a primer pair C, a primer pair D, a primer pair E, a primer pair F, a primer pair G, a primer pair H, a primer pair I, a primer pair J, a primer pair K and a primer pair L. According to the GeXP detection kit, shown by experiments, the primer group, a PCR (Polymerase Chain Reaction) reagent and the primer pairs, provided by the invention, are used for simultaneously differentiating and detecting avian influenza viruses, H5, H7 and H9 subtype avian influenza viruses, duck hepatitis viruses, duck plague viruses, duck flaviviruses, newcastle disease viruses, egg drop syndrome viruses, muscovy duck reoviruses, muscovy duck parvoviruses and duck circoviruses and are good in specificity and high in sensitivity. The detection kit, which is simple and convenient and is high in flux, and a detection system are provided for the detection on common major duck infectious disease pathogens, so that the practical needs are better met, and application prospects are broad.

The invention relates to a vaccine composition. The vaccine composition comprises an immunological dose of egg drop syndrome virus Fiber protein or an immunological dose of a live vector containing arecombinant egg drop syndrome virus Fiber protein gene, and a veterinary acceptable carrier. The vaccine composition can effectively protect the occurrence of egg laying of chickens, and can completely prevent against wild strains of egg drop syndrome virus in different regions.

The invention discloses a triple fluorescent quantitative PCR detection primer, kit and method for duck Tembusu virus, egg drop syndrome virus and H9 subtype avian influenza virus. The detection primers for duck Tembusu virus, egg drop syndrome virus and H9 subtype avian influenza virus are respectively shown in SEQ ID NO.1-6. The detection primer and kit of the present invention can detect EDSV, H9 AIV and DTMUV simultaneously, and do not interact with duck plague virus, goose parvovirus, muscovy duck parvovirus, Escherichia coli genomic DNA, duck hepatitis A virus type 1, and duck hepatitis A virus type 3 , Muscovy duck reovirus and Newcastle disease virus RNA cross-reacts, and the detection limits for EDSV, H9 AIV and DTMUV nucleic acids are 8.0, 4.8 and 1.3 copies respectively, which can be used for the qualitative and quantitative analysis of EDSV, H9 AIV and DTMUV Quantitative detection.

The invention provides a nucleotide sequence represented by SEQ ID NO. 2 or an Avian egg drop syndrome virus tFiber protein fragment encoded by a degenerate sequence of the nucleotide sequence and a vaccine composition prepared from the protein fragment. The fragment has good immunogenicity, can generate a good immunization effect in case of a low immunization amount and has the protective rate of 100%, the immunogenicity is better than that of a Fiber full-length protein, and a combined vaccine can be prepared from the fragment and a variety of antigens so as to generate complete protection on chickens and ducks.

Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The kit can detect simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.

The invention provides a method for detecting egg drop syndrome viruses and a kit for the method. By means of sequencing and comparing egg drop syndrome virus genes, a genetic marker for detecting the egg drop syndrome viruses, a real-time fluorescent quantitative PCR (polymerase chain reaction) primer and a probe are provided, and nucleotide sequences are respectively showed as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention further provides a real-time fluorescent quantitative PCR method for detecting the egg drop syndrome viruses and the detecting kit. The minimum detectable amount of the detection method reaches 10 copy / muL, the method has a good linear relation within a 1.0X102-1.0X108 copy / muL detection range, and R2 equals to 0.992. The method has the advantages of accurate detection, high sensitivity and specificity, simplicity, convenience and speediness, and has good specimen detection ability.

The invention discloses a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively vaccinating Newcastle disease virus ZM10 strain, infectious bronchitis virus M41 strain and H9 subtype avian influenza virus S2 strain in a susceptible chicken embryo, and collecting an infected chicken embryo solution; vaccinating egg drop syndrome virus AV-127 strain in a susceptible duck embryo, and collecting an infected duck embryo solution; and concentrating the collected virus solutions, inactivating, blending with an oil adjuvant, and emulsifying. The quadruple vaccine provided by the invention can be used for preventing diseases caused by Newcastle disease viruses, infectious bronchitis viruses, egg drop syndrome viruses and avian influenza (H9 subtype) viruses to achieve the effect of preventing four diseases simultaneously and reduce immune cost and immune stress reaction.