30 results about "Egg drop syndrome virus" patented technology

The invention discloses a GeXP (Gene Expression Profiler) detection kit for differentiating 11 kinds of duck viral diseases. The invention provides a GeXP detection primer group for identifying or assisting to identify duck infectious disease pathogens, wherein the primer group consists of a primer pair A, a primer pair B, a primer pair C, a primer pair D, a primer pair E, a primer pair F, a primer pair G, a primer pair H, a primer pair I, a primer pair J, a primer pair K and a primer pair L. According to the GeXP detection kit, shown by experiments, the primer group, a PCR (Polymerase Chain Reaction) reagent and the primer pairs, provided by the invention, are used for simultaneously differentiating and detecting avian influenza viruses, H5, H7 and H9 subtype avian influenza viruses, duck hepatitis viruses, duck plague viruses, duck flaviviruses, newcastle disease viruses, egg drop syndrome viruses, muscovy duck reoviruses, muscovy duck parvoviruses and duck circoviruses and are good in specificity and high in sensitivity. The detection kit, which is simple and convenient and is high in flux, and a detection system are provided for the detection on common major duck infectious disease pathogens, so that the practical needs are better met, and application prospects are broad.

Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The can kit detect, simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.

The invention relates to a vaccine composition. The vaccine composition comprises an immunological dose of egg drop syndrome virus Fiber protein or an immunological dose of a live vector containing arecombinant egg drop syndrome virus Fiber protein gene, and a veterinary acceptable carrier. The vaccine composition can effectively protect the occurrence of egg laying of chickens, and can completely prevent against wild strains of egg drop syndrome virus in different regions.

Belonging to the field of traditional Chinese medicines, the invention particularly relates to a Chinese medicinal composition treating a duck Tembusu virus. The Chinese medicinal composition is prepared from the following raw materials by weight: 45g of Schizonepeta, 30g of radix sileris, 25g of notopterygium roots, 25g of radix angelicae biseratae, 30g of radix bupleuri, 25g of Radix Peucedani, 30g of fructus aurantii, 45g of Poria cocos, 30g of Platycodon grandiflorum, 25g of Ligusticum wallichii, 15g of liquorice, 15g of mint, 30-50g of Venenum Bufonis, 40-80g of radix isatidis, 50-100g of folium artemisiae argyi, 30-50g of Polygonum multiflorum, 40-80g of hawthorn, and 40-80g of dried tangerine peel. The Chinese medicinal composition provided in the invention has very good efficacy on poultry egg laying decrease, inappetence, depression and diarrhea caused by the duck Tembusu virus, an influenza virus and an egg drop syndrome virus, and especially has significant efficacy on sudden egg drop syndrome caused by duck Tembusu virus infection.

The invention discloses a triple fluorogenic quantitative PCR detection primer, kit and method of the duck tembusu virus disease, the egg drop syndrome virus and the H9 subtype avian influenza virus. The detection primer for the duck tembusu virus disease, the egg drop syndrome virus and the H9 subtype avian influenza virus is shown in SEQ ID NO.1-6. The detection primer and kit can detect the EDSV, the H9 AIV and the DTMUV at the same time. No cross reaction with duck plague virus, goose parovovirus, muscovy duck parvovirus and escherichia coli genomic DNA and duck hepatitis A virus 1, duck hepatitis A virus 3, muscovy duck reovirus and newcastle disease virus RNA exists. The lower limits of detection for EDSV nucleic acid, H9 AIV nucleic acid and DTMUV nucleic acid are 8.0 copies, 4.8 copies and 1.3 copies respectively. The detection primer, kit and method can be used for qualitative and quantitative detection for the EDSV, the H9 AIV and the DTMUV.

The invention relates to a vaccine composition. The vaccine composition comprises an immunological dose of egg drop syndrome virus Penton protein or an immunological dose of a live vector containing arecombinant egg drop syndrome virus Penton protein gene, and a veterinary acceptable carrier. The vaccine composition can effectively protect the occurrence of egg laying of chickens, and can completely prevent against wild strains of egg drop syndrome virus in different regions.

The present disclosure provides a vaccine composition comprising an immune amount of Fiber protein of egg drop syndrome virus or an immune amount of a live vector recombined with gene of the Fiber protein of egg drop syndrome virus and a veterinarily acceptable carrier.

The invention discloses a triple fluorescent quantitative PCR detection primer, kit and method for duck Tembusu virus, egg drop syndrome virus and H9 subtype avian influenza virus. The detection primers for duck Tembusu virus, egg drop syndrome virus and H9 subtype avian influenza virus are respectively shown in SEQ ID NO.1-6. The detection primer and kit of the present invention can detect EDSV, H9 AIV and DTMUV simultaneously, and do not interact with duck plague virus, goose parvovirus, muscovy duck parvovirus, Escherichia coli genomic DNA, duck hepatitis A virus type 1, and duck hepatitis A virus type 3 , Muscovy duck reovirus and Newcastle disease virus RNA cross-reacts, and the detection limits for EDSV, H9 AIV and DTMUV nucleic acids are 8.0, 4.8 and 1.3 copies respectively, which can be used for the qualitative and quantitative analysis of EDSV, H9 AIV and DTMUV Quantitative detection.

The invention discloses an indirect ELISA (Enzyme-linked Immuno Sorbent Assay) kit for detecting a blood serum 8 type avian adenovirus antibody based on spike glycoprotein F. The indirect ELISA kit comprises an ELISA enzyme-linked plate which is coated with expression protein of blood serum 8 type avian adenovirus spike glycoprotein gene F, positive and negative controls, an HRP (Horseradish Peroxidase) labeled rabbit anti-chicken secondary antibody, a diluting solution, a color developing solution, a washing solution and a stopping solution. The ELISA kit disclosed by the invention can be used for specifically detecting a FAdV-8 antibody and does not react with anti-influenza virus blood serum, anti-Marek's virus blood serum, anti-Newcastle disease virus blood serum, anti-chicken infectious anemia virus blood serum, reticuloendotheliosis virus, ant-blood serum 4 type avian adenovirus blood serum, anti-egg drop syndrome virus blood serum and SFP chicken blood serum. Therefore, the kitdisclosed by the invention has good FAdV-8 specificity and can be used for FAdV-8 infection and immunization status epidemiological surveys.

The invention discloses a preparation method of an antigenic protein, comprising: respectively cloning the coding genes of codon-optimized recombinant avian egg drop syndrome virus Core protein, Fiber protein, Penton protein and Hexon protein into multiple recombinant shuttle vectors; Respectively transform the multiple recombinant shuttle vectors into competent host cells to obtain multiple recombinant baculovirus vectors; respectively transfect insect cells with the multiple recombinant baculovirus vectors to obtain recombinant baculoviruses; Co-infect insect cells to automatically assemble into VLPs in the cells and express antigenic proteins. The invention also discloses an immune composition containing the antigenic protein, which can be used to prepare a genetically engineered subunit vaccine of the avian egg drop syndrome virus. The structure, immunogenicity and function of the vaccine are similar to those of the natural protein, and the expression level is High, strong immunogenicity, no pathogenicity to animals, and can be prepared by large-scale serum-free suspension culture in a bioreactor, and the production cost is low.

The invention provides a nucleotide sequence represented by SEQ ID NO. 2 or an Avian egg drop syndrome virus tFiber protein fragment encoded by a degenerate sequence of the nucleotide sequence and a vaccine composition prepared from the protein fragment. The fragment has good immunogenicity, can generate a good immunization effect in case of a low immunization amount and has the protective rate of 100%, the immunogenicity is better than that of a Fiber full-length protein, and a combined vaccine can be prepared from the fragment and a variety of antigens so as to generate complete protection on chickens and ducks.

The present disclosure provides a vaccine composition comprising an immune amount of Fiber protein of egg drop syndrome virus or an immune amount of a live vector recombined with gene of the Fiber protein of egg drop syndrome virus and a veterinarily acceptable carrier.

Provided herein is a GeXP detection kit for identification of 11 kinds of duck virus diseases. The detection kit includes a primer set for identifying or auxiliarily identifying pathogens of duck communicable diseases, including one or more of primer pair A, primer pair B primer pair C, primer pair D, primer pair E, primer pair F, primer pair G, primer pair H, primer pair I, primer pair J, primer pair K and primer pair L. The kit can detect simultaneously avian influenza virus, subtype H5, H7 and H9 of avian influenza virus, duck hepatitis virus, duck enteritis virus, duck Tembusu virus, Newcastle disease virus, egg drop syndrome virus, Muscovy duck reovirus, Muscovy duck parvovirus and duck circovirus with the primer set, PCR reagent or primer pairs provided in the present invention.

The invention provides a method for detecting egg drop syndrome viruses and a kit for the method. By means of sequencing and comparing egg drop syndrome virus genes, a genetic marker for detecting the egg drop syndrome viruses, a real-time fluorescent quantitative PCR (polymerase chain reaction) primer and a probe are provided, and nucleotide sequences are respectively showed as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The invention further provides a real-time fluorescent quantitative PCR method for detecting the egg drop syndrome viruses and the detecting kit. The minimum detectable amount of the detection method reaches 10 copy / muL, the method has a good linear relation within a 1.0X102-1.0X108 copy / muL detection range, and R2 equals to 0.992. The method has the advantages of accurate detection, high sensitivity and specificity, simplicity, convenience and speediness, and has good specimen detection ability.

The invention discloses a quadruple inactivated vaccine for preventing chicken diseases, which is prepared by respectively vaccinating Newcastle disease virus ZM10 strain, infectious bronchitis virus M41 strain and H9 subtype avian influenza virus S2 strain in a susceptible chicken embryo, and collecting an infected chicken embryo solution; vaccinating egg drop syndrome virus AV-127 strain in a susceptible duck embryo, and collecting an infected duck embryo solution; and concentrating the collected virus solutions, inactivating, blending with an oil adjuvant, and emulsifying. The quadruple vaccine provided by the invention can be used for preventing diseases caused by Newcastle disease viruses, infectious bronchitis viruses, egg drop syndrome viruses and avian influenza (H9 subtype) viruses to achieve the effect of preventing four diseases simultaneously and reduce immune cost and immune stress reaction.

The invention discloses a duplex fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) detection kit for duck tembusu virus and egg drop syndrome virus. The detection kit comprises a primer group and a probe group, wherein the primer group comprises primers 1-4, which have base sequences as shown in SEQ. ID. No. 1 and 2, as well as SEQ. ID. No. 4 and 5 in a sequence table respectively; the probe group comprises a probe A and a probe B, which have the base sequences as shown in SEQ. ID. No. 3 and 6 in the sequence table respectively. Experiments prove that the detection kit disclosed by the invention has the advantages of short detection time, high detection sensitivity and strong specificity, and can realize simultaneous detection and identification of two pathogens of duck tembusu virus and egg drop syndrome virus and quantify the corresponding pathogen content, so that the detection kit can be used for evaluating curative effects of duck tembusu virus and egg drop syndrome virus vaccines and medicaments and researching pathogenic mechanisms and other aspects, and further has important significance in prevention and treatment of duck tembusu virus and egg drop syndrome virus.