Egg drop syndrome vaccine and preparation method thereof

A technology for egg production decline and syndrome, which is applied in the field of poultry vaccines, can solve the problems of low antibody levels and achieve high virus titers, clear cell sources, and fast and simple methods

Pending Publication Date: 2020-08-25
成都史纪生物制药有限公司
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Problems solved by technology

It has also been reported that EB66 cells were used to prepare chicken egg production drop syndrome vaccine, but the virus titer was around 17log2, and the immune antibody level after vaccination was between 10-12log2, and the antibody level was still relatively low

Method used

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  • Egg drop syndrome vaccine and preparation method thereof
  • Egg drop syndrome vaccine and preparation method thereof
  • Egg drop syndrome vaccine and preparation method thereof

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preparation example Construction

[0024] The present invention proposes a preparation method of chicken egg drop syndrome vaccine as described above, comprising the following steps: first resuscitating and culturing the retinal cells of fully suspended duck embryos, and then inoculating chicken egg drop syndrome virus (K-11 strain) Inoculate into cultured fully suspended duck embryonic retinal cells, add DMEM medium at the same time for cultivation, then collect virus and freeze-thaw. The preparation method is very simple, and the whole suspension duck embryo retinal cells are used at low cost, and the prepared vaccine has high potency and good immune effect.

[0025] Further, in some embodiments of the present invention, the recovery and cultivation of the above-mentioned fully suspended duck embryo retinal cells include the following steps: taking out the fully suspended duck embryo retinal cells from the liquid nitrogen tank, melting them rapidly in a water bath at 37°C, and then adding 200 g Centrifuge for...

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Abstract

The invention relates to the technical field of poultry vaccine and specifically relates to an egg drop syndrome vaccine and a preparation method thereof. The egg drop syndrome vaccine includes the inactivated antigen solution of eggs drop syndrome virus and an vaccine adjuvant. According to the invention, the antigen solution used for the vaccine is of high HA titre and low cost. The preparationmethod of the egg drop syndrome vaccine includes the following steps: thawing and culturing full suspended duck-embryo retinal cells, inoculating the cultured full suspended duck-embryo retinal cellswith the eggs drop syndrome virus (K-11) and adding a DMEM culture medium at the same time to perform culturing, and then obtaining virus and performing freeze-thawing. According to the invention, thevaccine prepared by the method is of higher titre and lower cost.

Description

technical field [0001] The invention relates to the technical field of poultry vaccines, in particular to a method for preparing a chicken egg production drop syndrome vaccine and the vaccine. Background technique [0002] Eggs drop syndrome (EDS for short) is a common infectious disease of laying hens caused by Eggs drop syndrome virus (EDSV for short), although the fatality rate of the disease is low , but the egg production of infected chickens will be severely reduced, and the egg quality will be affected. Affected chickens mainly show symptoms such as mass drop in egg production rate, abnormal eggshell, deformed egg body, and poor egg quality. The disease can reduce the laying rate of laying hens by 30-50%, the breakage rate of eggs reaches 40%, and shellless eggs and soft-shell eggs can reach 15%, causing serious economic losses to the chicken industry. [0003] At present, the production of vaccines related to chicken egg drop syndrome in my country mainly adopts in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/235A61K39/39A61P31/20C12N7/00C12R1/93
CPCA61K39/12A61K39/39A61P31/20C12N7/00A61K2039/555A61K2039/5252C12N2710/10234C12N2710/10251A61K2300/00
Inventor 邢刚丁莉李成山岳丰雄左榕琳肖倩潘倩
Owner 成都史纪生物制药有限公司
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