Primer group for amplifying pathogenic nucleic acid, construction method of pathogenic nucleic acid detection library and pathogenic detection method

Abstract

The invention relates to a primer group for amplifying pathogenic nucleic acid, a construction method of a pathogenic nucleic acid detection library and a pathogenic detection method. The primer groupcomprises at least one pair of multiple amplification primers, and the multiple amplification primers comprise a forward primer and a reverse primer, wherein the structure of each of the forward primer and the reverse primer comprises a universal primer amplification structure sequence, a sample label sequence and a pathogenic nucleic acid specific binding sequence. The universal primer amplification structure sequence is used for amplifying a universal primer, the sample label sequence is used for identifying the source of a sample, and the pathogenic nucleic acid specific binding sequence is used for specifically binding the target region of a pathogenic nucleic acid. According to the invention, a multiple amplification technology is adopted to enrich a target gene sequence of the pathogenic nucleic acid; the source of the sample can be determined by connecting the sample label sequence with the sample; and meanwhile, library preparation by mixing a plurality of samples together isrealized to improve the detection flux.

Description

technical field [0001] The invention relates to the technical field of pathogen detection, in particular to a primer set for amplification of pathogenic nucleic acid, a method for constructing a pathogenic nucleic acid detection library, and a pathogen detection method. Background technique [0002] With the advancement of high-throughput sequencing technology, sequencing service objects and application segments continue to expand, and the market size of high-throughput sequencing continues to increase. High-throughput sequencing technology has entered into rapid development in the detection of pathogenic microorganism typing, pathogenic microorganism identification and bacterial drug resistance monitoring, and has broad application prospects. [0003] (1) Type detection of pathogenic microorganisms, such as human papillomavirus nucleic acid type detection. Cervical cancer is the second most common cancer in women worldwide, and its incidence is mainly related to the persis...

AI Technical Summary

Problems solved by technology

In addition, Life provides a multiplex PCR amplification sequencing detection technology covering 12 bacteria and 18 drug-resistant genes. This technology has high detection sensitivity and is applicable to a wide range of sample types, but the detection cost is expensive

[0009] In summary, in the process of expanding the market for pathogenic microorganism typing detection, pathogenic microorganism identification and bacterial drug resistance monitoring, some of the existing technologies are expensive to detect and some are complicated to operate; and there are generally shortcomings such as insufficient detection throughput, which are not suitable for large-scale Crowd screening and testing are far from meeting the growing market testing needs

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