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New variety cultivation method for hypocotyls mutation of actinidia arguta embryos by ethyl methane sulfonate (EMS) induction

A technology of kiwifruit jujuba and a cultivation method, which is applied in the field of EMS-induced hypocotyl mutation of kiwifruit seed embryos, can solve the problems of variety uncertainty, fruit shape, quality difference, etc., achieve high mutation rate, The effect of high dominant mutation and good genetic stability of mutant

Active Publication Date: 2020-06-23
TONGHUA NORMAL UNIV +1
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Problems solved by technology

[0004] In order to solve the above-mentioned kiwifruit breeding by hybridization, genetic diversity and cultivation polymorphism are prone to occur, resulting in differences in fruit shape and quality in different regions of the same variety, resulting in uncertainty of the variety, causing growers and variety rights holders disputes and other issues, the present invention provides a new variety breeding method for EMS-induced mutation of the hypocotyl of Actinidia jujuba seed embryo

Method used

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  • New variety cultivation method for hypocotyls mutation of actinidia arguta embryos by ethyl methane sulfonate (EMS) induction
  • New variety cultivation method for hypocotyls mutation of actinidia arguta embryos by ethyl methane sulfonate (EMS) induction
  • New variety cultivation method for hypocotyls mutation of actinidia arguta embryos by ethyl methane sulfonate (EMS) induction

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Embodiment Construction

[0041] The technical solution in the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0042] Such as figure 1 and 2Shown, a kind of EMS induces the new variety cultivation method of the hypocotyl mutation of Actinidia jujuba seed embryo, comprising the following steps:

[0043] 1 Materials and methods

[0044] 1.1 Material selection and pretreatment

[0045] In mid-to-late September, select the mature fruit of wild kiwifruit (the fruit is plump, deformed and soft), wash the seeds in the fruit with clean water and leave the mature seeds at the bottom of the water, and then mix the mature seeds with river sand at a ratio of 1:3. Keep the relative humidity of 75% with the river sand mixture, and place it in an artificial climate box with a temperature of 18-20° C. and a r...

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Abstract

The invention belongs to the field of propagation technology of plants and particularly relates to a new variety cultivation method for hypocotyls mutation of actinidia arguta embryos by an ethyl methane sulfonate (EMS) induction. The new variety cultivation method for hypocotyls mutation of actinidia arguta embryos by EMS induction takes the hypocotyls of tara vine embryos as raw materials, utilizes a combination of EMS and a plant tissue cultivation technology, adopts methods of the embryogenic callus induction, the redifferentiation, and the embryoid body rejuvenation and the like, a new type artificial cultivation variety of the actinidia arguta with high independence of each individual plant , high purity genetic information of each individual plant, and clear source of each individual plant and the like is obtained, which has an important protection and ecological significance for expansion of the population of the actinidia arguta, and the method can be further applied to construction of a mutant library of the actinidia arguta and the new type artificial cultivation variety of the actinidia arguta can be directly used as a variety strain for cultivation and variety certification.

Description

technical field [0001] The invention belongs to the technical field of new plant varieties, and in particular relates to a method for cultivating a new variety by EMS-induced mutation of the hypocotyl of the kiwifruit seed embryo. Background technique [0002] Actinidia arguta (Sieb.et Zucc.) Planch.ex Miq., also known as Actinidia arguta (Sieb.et Zucc.) Planch.ex Miq. , vine pear, small foreign peach, etc., are deciduous vines of the genus Actinidia in the family Actinidiaceae. The fruit is edible, has high nutritional value, contains a lot of vitamin C, starch, pectin, etc., and can be processed into jam, juice, preserved fruit, canned food, wine making or used to make cakes, candies and other foods. It can also be used medicinally, and has the functions of strengthening, antipyretic, invigorating the stomach, and hemostasis; the root and root bark have certain therapeutic and inhibitory effects on digestive tract cancer; the oil content of the seeds is 35.62%, which is a...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/06A01H1/04
CPCA01H4/008A01H1/06A01H1/04
Inventor 顾地周刘宇苹朱俊义尹春丽廖卓候智文
Owner TONGHUA NORMAL UNIV
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