Method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis

A technology for chicken Newcastle disease virus and egg production decline, which can be used in antiviral agents, virus antigen components, pharmaceutical formulations, etc.

Inactive Publication Date: 2012-10-03
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chickens with acute disease, especially breeder chickens, often due to slow growth or stagnation, weight loss, low feeding utilization rate and high death rate, etc., it brings huge economic losses to the chicken industry.
[0005] There are many varieties of commercialized products on the market at home and abroad, but they are all single traditional vaccines. Multiple use of vaccines, especially inactivated vaccines, not only increases the burden of manpower and material resources on the chicken farm, but also causes the injection stress of catching chickens many times. Can affect performance and increase susceptibility of flocks to disease
Coupled with the continuous mutation of virus strains in recent years, although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable.

Method used

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  • Method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis
  • Method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] (1) Antigen preparation and inspection of semi-finished products:

[0072] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0073] (1) Inoculate the virus seeds used for production, and use sterilized saline to make 10 -4 Dilute and inoculate 200 10-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinholes after inoculation, and continue to incubate at 37°C without turning the embryos.

[0074] (2) Incubation and observation After the chicken embryos were inoculated, the embryos were photographed once a day, and 9 chicken embryos that died 60 hours ago were discarded. Thereafter, the embryos were photographed once every 4-6 hours, and the dead embryos were taken out at any time until 96 hours, a total of 152 dead embryos were taken out, and all of them were taken out, with the air chamber upward, and cooled at 2-8°C for 12 hours.

[0075](3) Harvesting Take out the cooled chicken embryos and harvest the allantoic fluid of the chicken embryos ...

Embodiment 2

[0109] Finished product inspection - the laboratory trial-produced a batch of triple inactivated vaccine about 6000ml, the batch number is 2011001.

[0110] 1. character

[0111] Appearance Milky white emulsion.

[0112] Dosage form oil-in-water type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it will not spread.

[0113] Stability Draw 10ml of vaccine into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and the water phase precipitated at the bottom of the tube is 0.1ml, which meets the regulations.

[0114] The viscosity is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and the viscosity value is 46cP, which meets the regulations.

[0115] 2. The filling volume inspection was carried out according to the method stipulated in the "Chinese Veterinary Pharmacopoeia". The results of the measurement of the three bottles were 251.8ml, 252.2ml, and 252.5ml, which...

Embodiment 3

[0131] We conducted a comparative test of the immune effect of the triple vaccine and the single vaccine of each component. The test method is as follows:

[0132] Use 60 21-day-old SPF chickens, 10 Newcastle disease, egg production drop syndrome, viral arthritis triple vaccine and Newcastle disease, egg production drop syndrome, viral arthritis single vaccine each 10, subcutaneously inject 0.5ml of vaccine / only, and the other 10 were not immunized as a control. Breeding in groups. Chickens in each group were blood collected before immunization and 7, 14, 21, 28, 35 days after immunization, serum was separated, and serum antibodies were measured. The results are compared in the table below.

[0133]

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Abstract

The invention relates to a method for producing triple inactivated vaccines for new castle disease, egg drop syndrome and avian viral arthritis. The inactivated vaccines are prepared by using new castle disease viruses, egg drop syndrome viruses and avian viral arthritis viruses as production strains and adopting an ultra strong concentration process and high-quality adjuvant; and the inactivated vaccines are quick in production of antibodies of immunized animals, high in effect and long in protection period, and reduce occurrence and propagation of epidemic diseases.

Description

technical field [0001] The invention relates to a method for preparing an oil emulsion vaccine for preventing Newcastle disease, egg production drop syndrome and viral arthritis in chickens, and belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate. It occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. [0003] Egg drop syndrome (EDS76) is a disease that was discovered in the late 1970s, characterized by decreased egg production rate, abnormal egg shell, deformed egg body, and poor egg quality in commercial laying hens and breeding hens. Characteristic viral infectious disease. Chicken flocks will not die after being infected with the virus, and no clinical symptoms will appear before laying eggs. When the egg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/235A61K39/17A61K39/12A61P31/14A61P31/20
Inventor 左青山李昌友郭伟伟宫晓王龙王红胡潇李金积
Owner YEBIO BIOENG OF QINGDAO
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