Three-dimensional culture hepatocyte device capable of simulating hepatic plate structure and culture method of hepatocyte

Abstract

The invention discloses a three-dimensional hepatocyte culture device capable of simulating a hepatic plate structure. The three-dimensional hepatocyte culture device capable of simulating the hepatic plate structure comprises a micro-fluidic chip, wherein a first main channel, a second main channel and a third main channel are formed in the micro-fluidic chip, connected in sequence linearly and intercommunicated; one end of the first main channel far from the second main channel, a junction between the first main channel and the second main channel and a junction between the second main channel and the third main channel extend symmetrically to form two branch channels; one end of the first main channel, far from the second main channel, extends linearly to form a branch channel simultaneously; material guide tubes, which are communicated with the corresponding branch channels, are arranged at the tail ends of the branch channels; conduction holes are formed in the material guide tubes; the material guide tubes are vertical to a plane on which the corresponding branch channels are arranged so as to be used for feeding raw materials which are needed for forming a liver plate structure. The invention also discloses a three-dimensional culture method of hepatocyte with the liver imitation plate structure by using the device. Through the manner, a three-dimensional growth environment of the hepatocyte in in-vivo liver, which is the same as the hepatic plate structure, is obtained in vitro.

Description

technical field [0001] The invention relates to the fields of liver tissue engineering and bioartificial liver, in particular to a three-dimensional culture device and a culture method for hepatocytes with a liver plate imitation structure. Background technique [0002] At present, liver transplantation is still an effective means of treating various end-stage liver diseases, but the serious shortage of donor liver sources and transplant rejection limit the wide application of liver transplantation. Therefore, bioartificial liver and liver tissue engineering aiming at liver function repair, replacement and reconstruction have become a new direction for the treatment of liver failure. [0003] One of the common problems of bioartificial liver and liver tissue engineering is how to simulate the three-dimensional culture microenvironment of hepatocytes in vitro. At present, the bioartificial liver has three-dimensional culture models such as hollow fiber type, multi-layer plat...

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Problems solved by technology

[0004] Although there are many three-dimensional culture models of hepatocytes, most of the culture models only realize the cultivation of hepatocytes in a three-dimensional growth environment, and do not really reproduce the culture of hepatocytes in vivo, and the functional state of hepatocytes is also different from that in vivo. hepatocytes are very different

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