Peony tissue culture method

A technique of tissue cultivation, peony, applied in horticultural methods, botanical equipment and methods, horticulture, etc.

Inactive Publication Date: 2014-03-12
阎立燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are only a small number of reports on the germplasm resources of Paeoniae genus and garden applications, tissue culture of gardenia varieties f

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1:

[0017] 1. Collection and processing of explant materials:

[0018] Choose the new young shoot apex leaves of the mother plant that grows luxuriantly and disease-free in June as the explant material, collect the whole wild or domestic peony plant and transplant it into a flower box, wash the leaves with water, and wait for the new leaves to grow. Cut the live stem tip from the mother plant and wash it with tap water for 15 minutes to remove the dust and fluff on the leaf surface. Put the stem tip in 70% ethanol and turn it over for 20 seconds. After removing the waxy substance on the leaf surface, rinse the leaf surface with water to remove the ethanol , Put it in 0.1% mercury liter solution and soak for 15min for disinfection, wash with sterile water and cut into 1cm 2 Small square, the back of the leaf is placed flat in the culture medium, 4 explants are placed in each bottle, the bottle cap is tightly closed, the bottle mouth is sealed with a preservation bag,...

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PUM

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Abstract

The invention discloses a peony tissue culture method, relates to a flower tissue culture method, and belongs to the field of plant tissue culture. The peony tissue culture method is characterized by comprising following steps: disinfection and cleaning for a peony explant, callus induction and differentiation, adventitious bud proliferation, adventitious bud induction and seedling hardening. The beneficial effects of the peony tissue culture method are that: large amount of tissue culture plants can be obtained, so that planting demands are met; the survival rate reaches 95.5%; the tissue culture plants can be cultivated in batch and also can be cultivated in the whole area; and pollution-free medicinal materials can be achieved two years earlier, so that the method is practical.

Description

technical field [0001] The invention relates to a flower tissue culture method, which belongs to the field of plant tissue culture, in particular to a peony tissue culture method. Background technique [0002] Peony is native to the mountainous areas of Qinling Mountains and Daba Mountains in western China. It is a small perennial deciduous shrub with slow growth and small plant type. Peony is a unique woody and precious flower in my country. It is known as "National Beauty and Heavenly Fragrance" and "King of Flowers". It has long been regarded as a symbol of wealth, auspiciousness and prosperity. Among peonies, Luoyang peony and Heze peony are the most famous. Peony root bark is used as medicine, named "Paeonol", and can be viewed as a bonsai plant. There are many colors of flowers, including pink, red, white, etc. The pink is crowned with powder, and the white is more famous for Jingyu. Peony not only has ornamental value, but also has high medicinal value. The root of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 阎立燕
Owner 阎立燕
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