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A method for distinguishing tilapia dorsal and tail muscles

A tilapia, muscle technology, applied in the field of immunoassay

Inactive Publication Date: 2015-08-05
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies that can solve this problem with protein detection methods have not been reported.

Method used

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  • A method for distinguishing tilapia dorsal and tail muscles

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Embodiment 1

[0019] (1)根据罗非鱼Coptodon rendalli BMP4基因氨基酸序列(Genbank登录号:BAC02605):MIPGNRMLMV ILICQVLLGE SNHASLIPEE GKKKVPGLQG RSAAQSHELL RDFEATLLHMFGLKRRPRPS RSATVPRYLL DLYRLQSGEA EEAGAHDIAF EYPERSASRA NTVRGFHHEEHMEQLHELDD GGPMPIRFMF NVSSIPEDEL LSSAELRLYR HQIDEAIADA ISDDQALHRINVYEVLKAPR PGQLITQLLD TRLVRHNASR WESFDVSPAV LRWTHERLPN YGLAVEVLHLNQTPRHQGRH VRISRSLHQE PGEDWEQLRP LLVTFGHDGK GHPLTRRTKR SPRQRGRKRNRNCRRHALYV DFSDVGWNDW IVAPPGYQAY YCHGECPFPL ADHLNSTNHA IVQTLVNSVNNNIPKACCVP TELSAISMLY LDEHDKVVLK NYQEMVVEGC GCR, designed a polypeptide from the above amino acid sequence, synthesized the polypeptide, and modified the polypeptide to obtain the tilapia BMP4 polypeptide, whose amino acid sequence is QVLLGE SNHASLIPEE GKc (where c is an increased cys, used for polypeptide coupling) ;

[0020] (2) On the basis of (1), the above-mentioned tilapia BMP4 polypeptide was used to immunize New Zealand white rabbits, and 5 booster immunizations were performed to prepare rabbit anti-BMP4 serum;

[0021] (...

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Abstract

The invention discloses a method of distinguishing a dorsal muscle and a tail muscle of Tilapia mossambica. The method is characterized in that a detected BMP (Bone Morphogenetic Protein) 4 antibody of Tilapia mossambica is used to detect the BMP4 proteins in the two muscle parts of Tilapia mossambica, and GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is used as a contrast to a target protein, and if proteins of 55KD are just detected, the muscle is the dorsal muscle of Tilapia mossambica.

Description

technical field [0001] The invention relates to a method capable of distinguishing tilapia back muscles and tail muscles, and relates to an immune analysis method. Background technique [0002] At present, consumers agree that among the back muscles and tail muscles of tilapia, the back muscles are fresh and tender, which is favored by consumers. In this way, in the process of tilapia production and breeding, the selection and formation mechanism of back muscles has become the primary problem that must be solved. Therefore, how to distinguish the back muscles of tilapia at the molecular level is particularly important. [0003] Molecular markers for identifying back muscles can be developed from tilapia back muscles and tail muscles, which can provide a breakthrough for research on the difference between back muscles and other muscles. At the same time, this molecular marker can be used for tilapia back muscles for breeding paths and methods. However, studies that can sol...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6803G01N2333/4603
Inventor 苏胜彦张成锋董在杰袁新华
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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