Array type multi-electrochemical isothermal amplification chip for detecting bacteria and preparation method thereof

Abstract

The invention belongs to the technical field of electrochemical detection and in particular relates to an array type multi-electrochemical isothermal amplification chip for detecting bacteria and a preparation method of the array type multi-electrochemical isothermal amplification chip. The chip consists of a laser-etched indium tin oxide (ITO) glass electrode substrate and a polydimethylsiloxane microchip. The amplification chip is prepared by taking the ITO glass and the polydimethylsiloxane as materials through the laser etching and micromachining technology. The chip is ordered in space array arrangement, and simultaneous detection for multiple nucleic acid targets is realized by utilizing space discrimination of an amplification signal; each amplification pool respectively contains a set of three-electrode system: a working electrode, a counter electrode and a reference electrode and is connected with an outside multi-channel electrochemical workstation, so that loop-mediated isothermal amplification (LAMP) reaction is carried out, and real-time detection is performed; the data of a real-time curve graph is processed to obtain a quantitative analysis result. The chip is easy to manufacture and convenient to operate, large-scale preparation is easily realized, and an effective scheme is provided for realizing simultaneous quantitative determination for multiple clinical pathogens by using the LAMP method.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, and in particular relates to an array type multi-electrochemical constant temperature amplification chip and a preparation method thereof. Background technique [0002] Upper respiratory tract infection refers to acute infections that occur in the respiratory tract, including common cold, influenza, nasopharyngitis, pharyngeal tonsillitis, and laryngitis. Most of them are self-limiting, but the incidence is relatively high. 80% of the pathogens of infection are viruses, followed by bacteria. And 78% of patients are diagnosed with antibiotics that are ineffective against viruses, which will seriously lead to the emergence of bacterial resistance. The clinical diagnosis is generally made by serology and bacterial culture, which are less sensitive and time-consuming. Therefore, timely qualitative and quantitative detection of bacteria that cause colds is of great significance for...

AI Technical Summary

Problems solved by technology

Traditional electrode preparation methods include electrode printing, electroplating, and electrodeposition techniques, but electroplating and electrodeposition techniques are cumbersome and not easy to commercialize, and the conductivity and reproducibility of printed electrodes are average.

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