Gas chromatography-mass spectrometry detection method of three very long chain fatty acids in human serum

A very long-chain fatty acid and gas chromatography technology, applied in the field of detection of very long-chain fatty acids, can solve the problems of complex, unfavorable and stable results of very long-chain fatty acids, and low extraction efficiency, so as to simplify the pretreatment process, save processing time, The effect of high extraction efficiency
CN103645263AActive Publication Date: 2014-03-19昆明金域医学检验所有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
昆明金域医学检验所有限公司
Publication Date
2014-03-19

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Abstract

The invention provides a gas chromatography-mass spectrometry detection method of three very long chain fatty acids in a human serum. The method comprises the following steps (1) carrying out pretreatment on a human serum sample to obtain a derivative product; (2) detecting the derivative product by using a gas chromatography-mass spectrometer; (3) carrying out qualitative analysis on each very long chain fatty acid in the human serum sample according to a total ion chromatogram and a mass spectrogram of the derivative product, and calculating the content of each very long chain fatty acid in the human serum sample according to a selective ion chromatogram, wherein each very long chain fatty acid comprises docosanoic acid, tetracosanoic acid and hexacosanoic acid. Compared with the background art, the pretreatment process disclosed by the invention is fast, sensitive and accurate.
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Description

technical field

[0001] The invention relates to the technical field of very long-chain fatty acid detection, in particular to a gas chromatography-mass spectrometry detection method for three very long-chain fatty acids in human serum. Background technique

[0002] The VLCFA (very-long-chain fatty acids) in the body mainly include docosanoic acid (C22:0), tetracosanoic acid (C24:0) and hexacosanoic acid (C26:0 ) 3 species, which can only undergo β-oxidation in peroxisomes. VLCFA mainly exists in the form of esters in the body, including triglycerides, phospholipids, glycolipids, etc., but a small part exists in free form or bound to serum albumin.

[0003] Determination of the content of VLCFA in serum can be used to assist in the diagnosis of genetic metabolic diseases related to suspected peroxisome metabolic disorders, including peroxisome biogenic disorders, X-linked adrenoleukodystrophy and peroxidase Physical function assessment, etc.

[0004] Currently, gas chromat...

Claims

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