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Effective polypeptide parts in Polybia spp. insect venom, and cardio-cerebral thrombosis use thereof

An effective part, a technology for cardiovascular and cerebrovascular diseases, applied in the field of medicine, can solve the problems of low level of industrial production support and failure to form large-scale production capacity, and achieve the effect of reducing cerebral edema and prolonging breathing time

Inactive Publication Date: 2014-03-26
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Effective polypeptide parts in Polybia spp. insect venom, and cardio-cerebral thrombosis use thereof
  • Effective polypeptide parts in Polybia spp. insect venom, and cardio-cerebral thrombosis use thereof
  • Effective polypeptide parts in Polybia spp. insect venom, and cardio-cerebral thrombosis use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 : Preparation of effective parts of Vespa orientalis venom polypeptide and coating agent

[0035] 1.1 Collection of crude venom from Vespa orientalis:

[0036] Vespa orientalis was collected from Pu'er City, Yunnan Province. The bee body specimen was identified as Polybia orientalis Saussure (Polybia orientalis Saussure) by Professor Mao Benyong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali College, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 9V , output automatic intermittent pulsating voltage; method of collecting wasp venom: place the poison collecting device in front of the hive, collect poison for the worker bees of the Vespa orientalis colony according to the routine poison c...

Embodiment 2

[0041] Example 2 .Preparation of effective parts of vespa venom polypeptide and hydrogel

[0042] 2.1 Collection of the crude venom of Vespa variegata:

[0043] The wasp was collected from Ruili City, Yunnan Province, and the bee body specimen was identified as Polybia stigma Smith by Professor Guo Yunjiao of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 12V , output automatic intermittent pulsating voltage; collection method of wasp venom: put the unpainted poison collection device in front of the hive, collect venom from the worker bees of the vespa colony according to the routine poison collection procedures. Turn on the power switch of the ...

Embodiment 3

[0048] Example 3 .Preparation of cataplasm from the active part of the Vespa vespisum venom in Sumatra

[0049] 3.1 Collection of crude venom from Heterogastricus Sumatran:

[0050] Vespa Sumatran was collected from Menghai, Yunnan Province. The bee specimen was identified as Polybia sumatrensis Saussure by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 9V, Output automatic intermittent pulsating voltage; The wooden frame is painted red with red paint in advance; The wooden frame is pre-smeared 20 milliliters concentration is the honeycomb and honeycomb body extract volatile solution (prep...

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Abstract

The invention relates to effective polypeptide parts in Polybia spp. insect venom, and a cardio-cerebral thrombosis use thereof. The effective parts are obtained through a step of dialyzing crude Polybia spp. insect venom through a 25KDa dialysis membrane, and a step of freeze-drying or reduced pressure concentration drying of the above obtained dialysis liquid. A series of pharmacodynamic experiments verify that the effective biotoxin polypeptide parts and transdermal absorption preparations thereof can substantially control the cerebral thrombus, can recover the mobility of cerebral palsy animals, can mitigate the ischemic brain injury, have stronger pharmacodynamic performances than first-line medicines comprising plavix, Xingnaojing and the like, and can be expected to be used for preparing innovative biochemical medicines for preventing and treating cardiovascular and cerebrovascular diseases in order to prevent and treat ischemic cardiocardiovascular diseases, such as myocardial infarction, cardiovascular thrombus, cerebral thrombus, cerebral infarction, acute / chronic cerebral apoplexy, and sequelae caused by cerebral infarction injury, like lateral hemiplegia, hemidysesthesia, hemiopia, facial and lingual paralysis, aphasia and general paralysis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to a preparation method and a medical application of an effective fraction of a polypeptide in Polybia spp. insects. The effective part is obtained by dialysis of the crude venom of Vespa heterospira with a 25KDa dialysis membrane, and the eluate is freeze-dried or concentrated and dried under reduced pressure. Through a series of pharmacological and pharmacodynamic experiments in animals, the inventors found that the effective part of the polypeptide has the effect of significantly preventing and treating cerebral thrombosis, restoring the activity ability of cerebral palsy animals, and reducing ischemic brain damage, and can be expected to be used in the preparation of anti-cardiac and cerebrovascular diseases. Vascular diseases include drugs, health care products, medical devices and daily chemicals for ischemic cerebrovascular disease and ischemic cardiovas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/34A61K38/17A61P9/00A61P9/10A61P7/02
Inventor 杨永寿陆丽王建超王斌杨云川赵昱巫秀美
Owner DALI UNIV
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