Flow cell

Abstract

The invention provides a flow cell. Compared with a former flow cell, flow cells with different light path lengths can be disposed in a same optical system without reduction of light amount and problem of positional relationships on an optical axis. In the flow cell, where a light introducing member for introducing light for measurement into a linear capillary through which sample liquid flows is attached to one end of the capillary, and a light leading out member for leading light transmitted through the capillary while transmitting through the sample liquid flowing through the capillary out to the outside is attached to the other end, the light introducing member is a light waveguide inserted into the capillary, and the light leading out member is a window member attached to an opening at the other end of the capillary so the loss of the amount of light transmitted through the capillary can be suppressed, while it is possible for flow cells of which the optical path has a different length to be attached without causing a problem relating to the positional relationships in the optical system, such as an absorbance detector.

Description

technical field [0001] The present invention relates to a flow cell for irradiating measurement light while flowing a liquid to be measured, for example, when measuring the absorbance of a liquid. Background technique [0002] In an absorbance detection device used in a liquid chromatograph, etc., a liquid to be measured (hereinafter referred to as "sample liquid") is filled or continuously circulated in a container called a cell, and light from a light source is irradiated. To measure light, the intensity of light transmitted through the sample liquid is detected to obtain absorbance for each wavelength. In order to measure a trace amount of sample liquid with high sensitivity, it is necessary to make the cross-sectional area of ​​the cell small and the optical path length long. Therefore, conventionally, a kind of circulation chamber called "light pipe cell" or the like has been put into practical use. A linear capillary is used as the cell, a sample liquid flows in the i...

AI Technical Summary

Problems solved by technology

[0014] However, when it is desired to connect small chambers with different optical path lengths to the same optical system, in the above-mentioned small chamber structure, in the space-coupling structure at both ends, either position of the light entrance position or light exit position, or both positions At positions different from each other for each cell, causing the light to deviate from the optimum position of the optical axis

[0015] On the other hand, in the two-end optical waveguide structure, by changing the total length of the capillary and the insertion amount of the optical waveguide inserted into both ends of the capillary, the optical path length can be changed with the same state of the light entrance position and the light exit position. When using an optical waveguide, there is a loss of light quantity, so there is a problem that the light quantity decreases compared with the above-mentioned two-terminal spatial coupling structure.

Images