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Method for preparing crosslinked chitosan porous scaffold

A technology of cross-linking chitosan and porous scaffolds, which is applied in medical science, prosthesis, etc., can solve problems such as weak mechanical strength, achieve high porosity, wide application prospects, and reduce production and construction costs.

Inactive Publication Date: 2015-01-14
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In line with the advantages of convenience, easy access, taking from nature and using in nature, taking advantage of the similarity and compatibility between natural biological materials and human tissues, many researchers have chosen natural biological materials to prepare tissue engineering scaffold materials, but natural biological materials The problem of weak mechanical strength generally exists, which requires people to choose an effective cross-linking agent to improve its mechanical properties, and the cross-linking agent must also be non-cytotoxic

Method used

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  • Method for preparing crosslinked chitosan porous scaffold
  • Method for preparing crosslinked chitosan porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The methanol suspension of 2% chitosan and the methanol solution of silane coupling agent KH-560 were configured respectively in mass percent;

[0022] Blend the methanol suspension of chitosan and the methanol solution of silane coupling agent KH-560 at a mass ratio of 70:30, heat up to 70°C, stir for 3 hours, filter with suction, and wash with anhydrous methanol repeatedly. Until the unreacted silane coupling agent KH-560 is washed and dried in vacuum at 60°C for 12 hours, cross-linked chitosan is obtained;

[0023] Dissolving the above-mentioned cross-linked chitosan in 100g distilled water, preparing an aqueous solution of 1% cross-linked chitosan by mass percent, degassing to obtain an aqueous solution of cross-linked chitosan after deaeration;

[0024] Pour the above-mentioned degassed cross-linked chitosan aqueous solution into a 10 mL microcontainer, and place it in a freezer at -30°C for 12 hours after being flattened, and then transfer it to a freeze dryer for...

Embodiment 2

[0027] Same as Example 1, but the concentration of the methanol suspension of chitosan and the methanol solution of silane coupling agent KH-560 in step (1) all changes from 2% to 1%, and the others remain unchanged.

Embodiment 3

[0029] Same as Example 1, but the concentration of the methanol suspension of chitosan and the methanol solution of silane coupling agent KH-560 in step (1) all changes from 2% to 3%, and the others remain unchanged.

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Abstract

The invention belongs to the technical field of novel materials and in particular relates to a method for preparing a crosslinked chitosan porous scaffold. The method comprises the following steps: by taking a chitosan natural biomaterial as a raw material and an organic silane coupling agent KH-560 as a cross-linking agent, preparing the crosslinked chitosan porous scaffold which is excellent in mechanical property and consists of pure natural biomaterials by adopting a freeze drying method. The prepared crosslinked chitosan porous scaffold has the large pore diameter of 100-300 mu m and the high porosity of more than 90 percent, is in an open hole structure in which a large pore contains a small pore, and is suitable for cell implantation on the scaffold surface. Compared with a chitosan scaffold, the crosslinked chitosan porous scaffold has higher mechanical property; due to the added organic silane coupling agent, the cell proliferation and differentiation on the surface of the porous scaffold are promoted, and the affinity to biological cells is high.

Description

technical field [0001] The invention belongs to the technical field of new materials, and in particular relates to a preparation method of a cross-linked chitosan porous support. Background technique [0002] The emergence of tissue engineering has brought bright prospects for human health and life extension. The use of biomedical materials to prepare artificial substitutes to treat diseases can solve the problem that human organs need to be replaced due to defects or loss of function, but the materials are limited. When people put their energy into artificial tissues or organs, one of the keys is the preparation of three-dimensional porous scaffold materials for tissue engineering using cell culture. [0003] At present, porous scaffold materials for tissue engineering are mainly derived from some natural polymers and synthetic polymers. Natural polymers mainly include collagen, chitin, chitosan, hyaluronic acid, gelatin, alginate, chondroitin sulfate, etc.; synthetic poly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/56A61L27/58
Inventor 杨正龙
Owner TONGJI UNIV
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