Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Silk fibroin/alginate hydrogel material and preparation method thereof

A technology of alginate and silk fibroin, which is applied in medical science, prosthesis, etc., achieves the effects of abundant raw material resources, simple and easy preparation method and good biocompatibility

Inactive Publication Date: 2014-04-09
SUZHOU UNIV
View PDF1 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the silk fibroin composite hydrogel prepared by blending silk fibroin and alginate is expected to be an ideal tissue engineering scaffold for biomimetic extracellular

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Silk fibroin/alginate hydrogel material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of natural mulberry silk fibroin / sodium alginate hydrogel material comprises the following steps:

[0026] (1) Natural mulberry silk was degummed by boiling with 0.05% sodium carbonate solution for 30 min, and the pure silk fibroin fiber was obtained after repeated 3 times; the silk fibroin was dissolved in LiBr:C 2 h 5 OH:H 2 O=44:45:11 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 70±2°C to obtain a regenerated silk fibroin protein solution; after dialysis, filtration, and concentration, a regenerated silk fibroin protein solution with a mass fraction of 30.36% is obtained;

[0027] (2) Dissolve 20g of sodium alginate in 80mL of deionized water, stir magnetically for 1 hour, and let stand for 24 hours to prepare a sodium alginate solution with a mass fraction of 20%;

[0028] (3) Add sodium alginate solution to silk fibroin solution, wherein the mass ratio of silk fibroin to sodium alginate is 7:3, then magnetically stir...

Embodiment 2

[0032] (1) Natural tussah silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure tussah silk fibroin fiber was obtained after repeated 3 times; the silk fibroin was dissolved in LiSCN solution in a water bath at a constant temperature of 50±2°C Internal stirring and dissolving to obtain a regenerated silk fibroin solution; after dialysis and filtration, a regenerated silk fibroin solution with a mass fraction of 15.95% was obtained;

[0033] (2) Dissolve 10 g of potassium alginate in 90 mL of deionized water, stir magnetically for 1 h, and let stand for 6 h to prepare a potassium alginate solution with a mass fraction of 10%;

[0034] (3) Add the potassium alginate solution into the silk fibroin solution to ensure that the mass ratio of the silk fibroin and potassium alginate is 1:1, then magnetically stir for 2 hours, mix the solution evenly, adjust the pH value of the mixed solution to 4.0 with hydrochloric acid, and then , place the mixed ...

Embodiment 3

[0037] (1) Natural castor silk was degummed by boiling 0.05% sodium carbonate solution for 30 min, and obtained pure castor silk fibroin fiber after repeated 3 times; the silk fibroin was dissolved in lithium bromide / ethanol (40:60, mass Stir and dissolve in a water bath at a constant temperature of 70±2°C to obtain a regenerated silk fibroin protein solution; after dialysis and filtration, obtain a regenerated silk fibroin protein solution with a mass fraction of 5.37%;

[0038] (2) Dissolve 2.0 g of ammonium alginate in 98 mL of deionized water, stir magnetically for 1 h, and let stand for 2 h to prepare an ammonium alginate solution with a mass fraction of 2.0%;

[0039] (3) Add the ammonium alginate solution to the silk fibroin solution to ensure that the mass ratio of silk fibroin to ammonium alginate is 1:10, then magnetically stir for 1 hour, mix the solution evenly, adjust the pH value of the mixed solution to 6.0 with hydrochloric acid, and then , place the mixed solu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a silk fibroin/alginate hydrogel material and a preparation method thereof. The preparation method comprise the following steps: (a) preparing a silk fibroin solution; (b) preparing an alginate solution; (c) mixing the silk fibroin solution and the alginate solution according to a certain ratio, evenly stirring, adjusting the pH value of the mixed solution, allowing the mixed solution to stand still to carry reactions at a certain temperature so as to a generate jelly-like substance; wherein the mass ratio of the silk fibroin to the alginate is 99:1-1:99. The prepared silk fibroin/alginate hydrogel material overcomes the shortage of bad mechanical property of silk fibroin hydrogel materials; furthermore, the silk fibroin/alginate hydrogel material has a similar structure, composition and functions as those of the cell epimatrix, and is an ideal tissue engineering material.

Description

technical field [0001] The invention relates to a hydrogel material and a preparation method thereof, in particular to a silk fibroin / alginate hydrogel material and a preparation method thereof. Background technique [0002] Hydrogel is a kind of polymer functional material that integrates water absorption, water retention and controlled release. Its properties are similar to those of the extracellular matrix. After absorbing water, it can reduce friction and mechanical effects on surrounding tissues and improve the biological properties of the material. . Due to its unique water absorption, water retention and biomimetic properties, hydrogel materials can be used as drug sustained release agents, tissue engineering scaffold materials, etc. in the field of biomedicine. [0003] Hydrogels include natural hydrogels and synthetic hydrogels. Natural hydrogel materials are mainly collagen (fibrin, glycoprotein, etc.), hyaluronic acid, alginic acid, chitosan, etc.; synthetic hydr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08J3/075C08L89/00C08L5/04A61L27/26A61L27/22A61L27/20A61L27/52
Inventor 明津法左保齐
Owner SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com